===== Writing Matlab code for ImarisXT ===== Getting Imaris object is pretty similar (see previous notes). Found several links besides [[http://www.bitplane.com/go/products/imarisxt/xtensions/matlab| Bitplane site]]. **Aaron Christian Pontis Qu** http://www.scs2.net/home/index.php?option=com_content&view=article&id=46:qu-for-matlab&catid=34:qu&Itemid=55&showall=1 Pretty much of communication with Imaris. * Sending 4D stack (successful with 8 z-slices, 46 timepoints tiff hyperstack) * Retrieving spots detected in Imaris back to matlab (I waited 5 minutes but currently not responding... finally, I got all the spots, none-filtered. It took time probably because I did not filter). **How to write Matlab Code for ImarisXT: a Short Tutorial** http://sybil.ece.ucsb.edu/pages/imarisxt.html this is a bit outdated. ---- I will further try doing something like * set parameters for spot detection from Matlab or Java * do spot analysis in Imaris using above setting * fetch the results of detected spots from Imaris to Matlab or Java * evaluate the quality. Change spot detecton parameter * do spot analysis again with updated parameter. * this looping goes on until certain satisfactory value is achieved, walk through parameter space. For this, image stack itself stays in Imaris. Only parameter setting is exported from java/matlab to imaris, and analysis results are thrown back to matlab/java.