Table of Contents

TBI Summer Course, Biocity, Turku, Finland

Dates: Aug. 7 - 10, 2012

course web site

Lecture Slides

Slides: Image Analysis in Cell and Developmental Biology (14Mb)

Practical Textbooks

Click the link below and please find textbook pdf files.

Morphometry

Distributions

3D measurement of nucleus foci positions: Method using distance map is written in the following article.

Kinetics, FRAP

The study of colesterol depletion effects on ER exit dynamics is in the following paper.

I did not talk about FRAP. In addition to Leong's lecture, you could follow the lecture notes below (if you are interested in modeling).

Lecture Notes: FRAP internal course

Colocalization

Recommended review:

  1. Bolte S, Cordelières FP. A guided tour into subcellular colocalization analysis in light microscopy. Journal of microscopy. 2006;224(Pt 3):213–32. Available at: http://www.ncbi.nlm.nih.gov/pubmed/17210054.

For those interested in Colocalization tests:

  1. Steensel B van, Binnendijk EP van, Hornsby CD, et al. Partial colocalization of glucocorticoid and mineralocorticoid receptors in discrete compartments in nuclei of rat hippocampus neurons. Journal of cell science. 1996;109 ( Pt 4:787–92. Available at: http://www.ncbi.nlm.nih.gov/pubmed/8718670 [Accessed August 7, 2012].
  2. Manders EM, Stap J, Brakenhoff GJ, Driel R van, Aten J a. Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy. Journal of cell science. 1992;103 ( Pt 3:857–62. Available at: http://www.ncbi.nlm.nih.gov/pubmed/1478975.
  3. Fay FS, Taneja KL, Shenoy S, Lifshitz L, Singer RH. Quantitative digital analysis of diffuse and concentrated nuclear distributions of nascent transcripts, SC35 and poly(A). Experimental cell research. 1997;231(1):27–37. Available at: http://www.ncbi.nlm.nih.gov/pubmed/9056409 [Accessed August 7, 2012].
  4. Costes SV, Daelemans D, Cho EH, et al. Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophysical journal. 2004;86(6):3993–4003. Available at: http://www.ncbi.nlm.nih.gov/pubmed/15189895.
  5. Fletcher P a, Scriven DRL, Schulson MN, Moore EDW. Multi-image colocalization and its statistical significance. Biophysical journal. 2010;99(6):1996–2005. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2941013&tool=pmcentrez&rendertype=abstract [Accessed August 7, 2012].

Slides below might be helpful as well

Tracking

List of available tracking softwares could be found in the link below.

3D drift correction I mentioned in the talk is in Fiji.

... other info will be added later.