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EMBL BioImage Data Analysis

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Timeline of @cmci_

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RT @biomicugm: Topics: Biostatistics, computational evolutionary biology, computational genomics and proteomics, graph models, image analys…
About 4 days, 16 hours ago by: Kota Miura (@cmci_)

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RT @JustinMCrocker: We are looking for an interdisciplinary EIPOD postdoc to join our group @EMBL! Potential projects with @Eileen_Furlong,…
About 4 days, 16 hours ago by: Kota Miura (@cmci_)

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RT @BioImagingUK: This maybe of interest to the @NEUBIAS_COST @openmicroscopy communities #imageAnalysis #imagingScientist https://t.co/I5
About 4 days, 16 hours ago by: Kota Miura (@cmci_)

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RT @BioImagingUK: Software developer position on large image data analysis - working closely with the lab of @bayraktar_lab @sangerinstitut…
About 1 week, 2 days ago by: Kota Miura (@cmci_)

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RT @AssafZaritsky: Modeling the interplay of lamellipodial motion and the intercellular actomyosin cable during epithelial wound healing h…
About 1 week, 2 days ago by: Kota Miura (@cmci_)

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RT @jytinevez: @florianjug says be careful when using #CARE for intensity based quantification; CARE restoration is like a non linear trans…
About 1 week, 4 days ago by: Kota Miura (@cmci_)
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Algorithm FRAP Fiji ImageJ ImageJ Plugin ImageJ Plugin 3Dviewer Imaris Java Javascript Python R bias blog dokuwiki fiji google imagej java libraries matlab meetings neubias news papers python references software webadmin




CMCI weblog

The 3rd CMCI symposium, July 5th, 2013

We will have the third CMCI image analysis symposium on July 5th (Fri), 2013. See more details.

To be in R programming course

I do write some scripts in R but not really serious programming. As I am interested in advanced use of R, I will be in the following course organized by Wolfgang Huber.

http://www-huber.embl.de/courses/2013-advR/

The course seems to match with my stage, as it explains

This course is designed for users who have experience with writing R scripts, and who now want to advance one step further, into producing more durable and robust software projects and code that is usable by others.

As I always have had a plan to migrate FRAPcalc program from IgorPro to R and also add some more functionalities, this course should be the one I should attend… I thank Wolfgang for his initiative in making this course available. Besides, I am happy to see another EMBL centre being active!

BIAS 2013 wiki page

There will be “EMBL Master course on BioImage Data Analysis” in May, and I am preparing for this course with Christoph Moehl (Bonn) and Sebastien Tosi (Barcelona). For the course we want to be interactive so we now have a wiki for the course:

http://cmci.embl.de/shared/bias2013

We abbreviate the course title “BIAS 2013”. Registration deadline is in February, and we will be waiting for many applications!

ImgLib2, first test

I pretty much enjoyed ImageJ User and Developer conference 2012 in Luxemburg. Interesting people from different areas in science, very exciting to see how people are doing image processing and analysis. Some of live notes could be found in my time line. One of the great contribution for the third day was Bene Schmidt's work intseg_3D, combining user clicking and active contour fitting in 3D.

One of the core topic there was ImgLib2, the generic image typ behind ImageJ2. After coming back home, I tested ImgLib2 with the Jython code below.

Results were: Started testImgLib2.py at Sun Oct 28 16:41:57 CET 2012

  • ImgLib2 cursor 333 millisec
  • ImgLib2 for : 138 millisec
  • ImagePlus 152 millisec
  • ImageProcessor 114 millisec
  • Pixel Array 59 millisec

for loop using Img iterator is a bit faster than going through ImagePlus using classic nested for-loop. Even with the classic nested for-loop, using ImageProcessor was a bit faster than Img. The fastest is of course accessing pixels via pixel array. I only did this via ImagePlus, but there should be also some methods to extract an array from Img, which I did not test.

Addendum: See also proper benchmark in the following link in ImageJ2 site:

http://developer.imagej.net/imglib-benchmarks

Thanks to Albert Cardona for commenting on this.

# first trial with ImageLib2
# iterations

from net.imglib2.img import ImagePlusAdapter

imp = IJ.openImage("http://imagej.nih.gov/ij/images/blobs.gif")
img = ImagePlusAdapter.wrapReal(imp)

# accessing via ImagePlus
t3 = System.currentTimeMillis()
for i in range(imp.getHeight()):
	for j in range(imp.getWidth()):
#		print imp.getPixel(j, i)[0]
		imp.getPixel(j, i)[0]
				
t4 = System.currentTimeMillis()

# accessing via ImageProcessor
t5 = System.currentTimeMillis()
ip = imp.getProcessor()
for i in range(imp.getHeight()):
	for j in range(imp.getWidth()):
#		print ip.getPixelValue(j, i)
		ip.getPixelValue(j, i)
				
t6 = System.currentTimeMillis()

# ImgLib2 cursor
t1 = System.currentTimeMillis()
cursor = img.cursor()
cursor.fwd()
while cursor.hasNext():
	cursor.next()
#	print cursor.get()
	cursor.get()

t2 = System.currentTimeMillis()

t7 = System.currentTimeMillis()
for t in img:
#	print t.get()
	t.get()

t8 = System.currentTimeMillis()

# pixel array
t9 = System.currentTimeMillis()
pix = imp.getProcessor().getPixels()
for i in pix:
	i = i
#	print i

t10 = System.currentTimeMillis()	

print 'ImgLib2 cursor',(t2-t1), 'millisec'
print 'ImgLib2 for : ',(t8-t7), 'millisec'
print 'ImagePlus',(t4-t3), 'millisec'
print 'ImageProcessor',(t6-t5), 'millisec'
print 'Pixel Array',(t10-t9), 'millisec'
	

New Server

This site has migrated to a new server and also now running with the most recent Dokuwiki! You might not recognize this move and upgrade, but this could not have been done with helps and hackings by Agustin Villalba @ IT service. This Dokiwiki web application is running with sqlite3 (Dokuwiki does not deal with sqlite3 normally). Thanks to Agustin!

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Weblog Archive

blogtng/blogtop.txt · Last modified: 2016/05/24 05:46 (external edit)