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# Lecture Notes: FRAP internal course

April 20, 2010 @EMBL
Kota Miura

will be also further added by Sebastian Huet and Christian Tischer

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## Introduction

in vivo protein kinetics could be analyzed in two ways: measuring particular movement or averaged movement. By tracking labeled single protein molecules, we could estimate their diffusion and transport behavior. Such single molecule studies of membrane proteins, for example, enabled us to analyze how they are organized with their dynamics, such as boundary for movement constrained by membrane corrals. Motor protein moving along cytoskeletal tracks were analyzed in detail to know how they convert chemical energy into physical force. This was only possible by probing their singular movement and steps. While single particle tracking requires high-temporal and spatial resolution setup for analysis, analysis of averaged movement, measured by temporal changes in fluorescence intensity, could be achieved with larger spatial and temporal resolution (typically in micrometer scale).

Here, we focus on one of such averaged movement analysis technique: Fluorescence Recovery After Photobleaching (FRAP). We first start the explanation with a simpler case of monitoring averaged movement that does not need to bleach.

## Fluorescence intensity and Protein Dynamics

Increase in intensity at observed area could be measured to know the net increase in the protein at that region. To characterize this dynamics, we can apply traditional biochemical kinetics. Example case: Kinetics of VSVG protein accumulation to ER exit site.

<jsmath>{dI(t)\over dt}=k_{on}[VSVG_{free}]- k_{off}[VSVG_{ERES}]</jsmath>

Here,

• <jsm>k_{on}</jsm> is the binding rate of VSVG protein to ER exit site
• <jsm>[VSVG_{free}]</jsm> is the concentration of unbound VSVG protein
• <jsm>k_{off}</jsm> is the dissociation rate of VSVG protein from ER exit site
• <jsm>[VSVG_{ERES}]</jsm> is the density of VSVG protein bound to the ER exit site

During the initial phase of binding, when there is almost no VSVG protein bound to ER exit site, we can approximate the initial speed of the density increase at ERES site depends only on binding reaction: <jsm>k_{off}[VSVG_{ERES}]\simeq0</jsm>. \\Then

<jsmath> {dI(t)\over{dt}}=k_{on}[VSVG_{free}] </jsmath>

Since there are enough free VSVG, we consider that <jsm>[VSVG_{free}]</jsm> is constant, we are able to simply calculate the slope of initial increase of intensity, measure the free VSVG intensity and then calculate <jsm>k_{on}</jsm>.

For details, see Runz et al (2006).

## FRAP Simple Measures

Unlike the example shown above, dynamics of protein are not observable to eyes (through microscope) in many cases. Even though proteins are exchanging in system, the flux of protein constituting the system is not evident if the in/out flux of protein is steady and constant (e.g. liver). In such cases, we need to some how experimentally treat the system. One way is FRAP.
In FRAP, we bleach some population of fluorescence-labeled protein and evaluate the mobility of the protein. Typically we use confocal microscopy and bleach fluorescence of small area of the system by short pulse of strong laser beam and measure following changes in the fluorescence intensity at that bleached spot over time. Detail on these measurement protocol has been presented in Stefan and Yury's talks (link?). Here, we focus on how to analyze the curve we obtained through such measurements.

From measured temporal changes in the intensity at the bleached Region of Interest (this curve indeed is the Fluorescence Recovery After Photobleaching, FRAP) we can measure two parameters which represents speed of recovery, and fraction of molecules that is moving around in the system.

• Half Max
• Mobile-Immobile fraction

Fitting the curve to exponential equation eases us to calculate these parameters. Half-Max value (time) is rather qualitative value, but is a simple and straightforward index for comparing different systems.

## FRAP Measurements based on Modelling

FRAP curve reflects the mobility of proteins. In dilute solution of with single protein solute, mobility of protein could probably be considered as pure-diffusion. But in many cases, this is does not hold. The mobility is often affected by the system.

• reaction with other proteins
• geometry of the system, that constrains the mobility
• active transport process

By modeling how the mobility is (generate some hypothesis how the protein mobility is affected in the system), we can set up equation/s to hypothesize what is the bases of FRAP curve. To test the hypothesis, we fit the experimental curves with theoretical curve. By evaluating the goodness of fit, we can discuss which models would be the most likely hypothesis. If the fit is good, then we could know the value of biochemical parameters which governs the recovery curve.

Currently we have more-or-less standardized protocol to analyze FRAP curves. Starting with simple model of diffusion, we test the fit of different curves and proceed to more complex models. See next section for the protocol.

### Choosing models based on trial fittings

Maybe Sebastian's flow chart here.

### Diffusion Recovery

#### pure diffusion

Theoretical curve of the diffusion mediated fluorescence recovery was proposed by Soumpasis (1984) and has been widely used.
<jsmath> f(t)=e^{- \frac{\tau_D}{2t}}\left(I_{0}(\frac{\tau_D}{2t})+I_{1}(\frac{\tau_D}{2t})\right) </jsmath> This theoretical equation assumes:

• 2D
• circular (cylindrical) bleaching

when above equation could be fitted nicely (evaluated by goodness of fit, such as Pearson's coefficient r or gamma-Q value), one could calculate diffusion coeffecient by using the obtained <jsm>\tau_D</jsm> and radius of the circular ROI <jsm>w</jsm>.
<jsmath> D=\frac{w^{2}}{\tau_D} </jsmath>

For strip-ROI bleaching, empirical formula used by Ellenberg et al. (1997) could be used, and is also possible to use Gaussian curve fitting that Christian Tischer developed. For Christian's method, 100426FRAPgaussfit.

#### effective diffusion

(almost diffusion)

### Reaction Dominant Recovery

If molecule under study is binding/unbinding with other molecular species, FRAP curve is affected by these interactions. There are two cases on how these two events, diffusion and reaction, are combined in the curve. We could think of two cases.

1. Reaction-dominant recovery Cases when reaction binding rate (meaning “number of molecules that binds to the binding partner per second”) is much lower than the diffusion rate. In such cases, FRAP-bleached field will first become brighter due to diffusion, and then there will be a slower recovery of intensity due to none-bleached fluorescence exchange with the bleached fluorescence. In such cases, we could consider that the recovery curve is dominated by reaction since duration of diffusion-recovery phase is much shorter compared to reaction-recovery phase. In such case, these two phases are considered to be separable (call this reaction-dominant or diffusion uncoupled; this section). We even might not “see” the diffusion recovery phase, which requires high temporal resolution capturing.
2. Reaction-Diffusion Recovery Other cases would be when durations of diffusion-recovery phase and reaction-recovery phase are with comparable duration. Then recovery curve consists of a combination of fluorescence that came in with diffusion, and also by the binding of fluorescence molecule at the FRAP bleached field (“reaction-diffusion” or “diffusion-coupled” see the section below).

For a simple chemical reaction with singular type of interaction, we could again think of the reaction model that was already explained above, the first-order chemical reaction modeled as a compartment system (see figure right)
<jsmath> \frac {df(t)} {dt} = k_{on}[free] - k_{off}[bound] </jsmath> where

• <jsm>k_{on}</jsm> Binding constant
• <jsm>k_{off}</jsm> Dissociation constant
• <jsm>[free]</jsm> Density of free molecules
• <jsm>[bound]</jsm> Density of bound-molecules

We solve the differential equation <jsmath> f(t)=A(1-e^{- \tau t}) </jsmath> where

• <jsm>\tau = k_{on} + k_{off}</jsm>
• <jsm>A = \frac {k_{on}}{k_{on} + k_{off}}</jsm>

#### Reaction Dominant Recovery with Immobile Binding Partner

Next we modify above model to consider a situation a bit more frequently we see in cell biology. The protein we are analyzing is either freely diffusing in cytoplasm or bound to an immobile structure inside cell. We FRAP this structure, to know the kinetic constants of the protein interaction with the structure (e.g. microtubule binding protein, structure = microtubule) <jsmath> \frac {df(t)} {dt} = k_{on}[free][s] - k_{off}[bound] </jsmath> where

• <jsm>k_{on}</jsm> Binding constant
• <jsm>k_{off}</jsm> Dissociation constant
• <jsm>[free]</jsm> Density of free molecules
• <jsm>[s]</jsm> Density of immobile binding partner
• <jsm>[bound]</jsm> Density of bound-molecules

Since [s] is immobile and constant during experiment, we define <jsm>k*_{on}</jsm> as <jsmath> k*_{on}=k_{on}[s] </jsmath> in addition, density of free molecule in cytoplasm is almost constant so we assume <jsm>[free] = F</jsm> and does not change. We then solve <jsmath> \frac {df(t)} {dt} = k*_{on}F - k_{off}[bound] </jsmath> We get <jsmath> f(t)=1-Ce^{- \tau t} </jsmath> where

• <jsm>\tau = k_{off}</jsm>

… note that the shape of recovery curve now only depends on <jsm>k_{off}</jsm>

### Diffusion and Reaction combined Recovery

<jsmath> \frac{\partial [free]}{\partial t} = D_{free} \nabla ^2[free]-k_{on}[free][s]+k_{off}[bound] </jsmath> <jsmath> \frac{\partial [s]}{\partial t} = D_s \nabla ^2[s]-k_{on}[free][s]+k_{off}[bound] </jsmath> <jsmath> \frac{\partial [bound]}{\partial t} = D_{bound} \nabla ^2[bound]+k_{on}[free][s]-k_{off}[bound] </jsmath>

Since

• [s] is constant and immobile
• <jsm>k*_{on} = k_{on}[s]</jsm>
• <jsm>\frac{\partial [s]}{\partial t}=0 </jsm>
• bound molecules do not diffuse so <jsm>D_{bound}=0</jsm>

Then we solve only <jsmath> \frac{\partial [free]}{\partial t} = D_{free} \nabla ^2[free]-k*_{on}[free]+k_{off}[bound] </jsmath> <jsmath> \frac{\partial [bound]}{\partial t} = k_{on}[free][s]-k_{off}[bound] </jsmath>

We could solve this either analytically (Sprague et al, 2004) or numerically (Beaudouin et al, 2006). In the latter paper, calculation involves spatial context (on-rate was spatially varied; see also “geometry” section below).

#### Sprague Method

Analytical solution was made in Laplace transformed equation. <jsmath> \overline{frap(p)} = \frac 1 p - \frac{F_{eq}}{p}\left(1-2K_1(qw)I_1(qw)\right)\times\left(1+\frac{k*_{on}}{p+k_{off}}\right)-\frac C {p+k_{off}} </jsmath>

### Diffusion and Transport combined Recovery

We did not talk about this issue in the course, but there is another factor that could interfere with recovery curve in vivo: active transport. There is some trial on including this factor by Hallen and Endow (2009).

### Diffusion and Reaction, along with Spatial Context, Geometry

Since molecular behavior inside cell is constrained largely by structure and geometry of intracellular architecture, FRAP measured at single point within a cell does not always represent the biochemical characteristic of that molecule. In fact, functionality of protein molecule is determined not only by switching on/off, but is also regulated by the position of that molecule within cell. This means that spatial context should be included when interpreting the FRAP measurement.

Physical parameters such as Diffusion coefficient measured by FRAP is affected largely by geometrical constraint. Even if the geometry is rather simple, there are many obstacles in intracellular space which will cause longer time for molecules to reach from one point to the other. In such cases (which probably is frequently the case), estimated diffusion coefficient would calculated to be smaller than that of the “true diffusion coefficient”. Presence of such obstacles should be somehow taken into account. For this reason, estimation of Diffusion coefficient could be more precise if one analyzes the structural geometry of where the molecule is constrained. Such protocol would be especially important if Diffusion and Reaction coupled recovery curve since wrong estimates on Diffusion coefficient would end up in wrong reaction parameters as well.

Joel Beaudouin who did PhD study in the Ellenberg lab in the EMBL actually encountered such question in his project on nuclear protein study and solved the problem by using initial image frames of the FRAP experiment and let the molecule to diffuse by simulation, then fit the simulation with the experimental FRAP image sequence. Diffusion-reaction model was used and simulation was done (see above) using ODE solver to scan through the parameter space. Excellent idea. For more details, see Beaudouin et al. (2006). We will later add some protocol to this lecture notes on how to fit FRAP image sequence data using ODE solver (with help of Sebastian Huet). Joel's method was actually made into application called “Tropical” by a team in University of Heidleberg, but it seems that there is no direct link for downloading.

Other papers we could refer to are Sbalzarini et. al. (2005, 2006). In these papers, authors did two things in parallel: 3D reconstruction of ER membrane structure and FRAP of certain molecule moving around along ER membrane. Using the 3D structure they reconstructed, geometrical constraint on protein diffusion could be determined and include this constraint on the estimation of Diffusion coefficient.

## Pitfalls in FRAP Analysis

Refer to Mueller et. al.(2008). In their paper, they pointed out

• Shape of the FRAP ROI largely affect estimated value of biochemical rate constants(diffusion is important)
• Problems of fitting double exponential curve
• Initial condition (laser intensity profile) is important
• “blinding” of photomultiplier after the FRAP bleaching

## List of Tools for FRAP Analysis

### Basic

• ImageJ Plugins: Two available ImageJ plugins for FRAP does intensity measurement and normalization. Fitting module is not implemented.
• There is a good step-by-step protocol for measurements and analysis using MBF ImageJ bundle. You need to download the bundle from the linked site, and also ask for some plugins directly by email.
• Import data output from Zeiss, Leica, Olympus measurements and do FRAP fitting.
• Does measurement and fitting. Sprague et al. (2004) Reaction-Diffusion Full model is implemented.

### Two Advanced strategies: Analytical and Numerical Approaches

#### Analytical Approach

Sprague et. al. (explained above) is an example case of analytically solving the model for the fitting.

### ODE Simulation

Tropical

• Numerical analysis based on ODE. Spatial context.

General Solvers

• Joel and Sebastian uses this software for fitting ODE.

### Particle Simulation

Particle Simulation Packages

• Smoldyn
• GridCell
• MCell

Reviews, Protocols and textbooks

• McNally, J.G. (2008) Quantitative FRAP in analysis of molecular binding dynamics in vivo. Methods in Cell Biology 2008;85:329-51
• step-by-step protocol for analyzing FRAP. Flow chart for determining recovery model is useful.
• Sprague, B. L. and McNally, J. G.(2005). FRAP analysis of binding: proper and fitting. Trends Cell Biol 15, 84-91.
• Phair, R. D., Gorski, S. A. and Misteli, T. (2004). Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopy. Methods Enzymol 375, 393-414.
• Already a bit of out-dated but “Double normalization” introduced in this protocol is widely used.
• Jacquez, J. A. (1972). Compartmental analysis in biology and medicine: Elsevier
• If you want to study basics about calculation of kinetics.

Research Papers 