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documents:101021cbbpractical [2010/10/26 09:20] kotadocuments:101021cbbpractical [2016/05/24 12:46] (current) – external edit 127.0.0.1
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 ==== Methods: ==== ==== Methods: ====
  
-Drosophila EMbryo sequence was provided by Sasha Necakov @ Stefano De Renzis lab. We analyze movement of EB1 signal using three strategies with following steps: +Drosophila EMbryo sequence was provided by Sasha Necakov @ Stefano De Renzis lab. 
 + 
 +XY scale 
 +1 pixel = 0.266 micrometrer 
 + 
 +Time/Frame 
 + 
 +We analyze movement of EB1 signal using three strategies with following steps: 
  
   * Track them either manually or automatically.   * Track them either manually or automatically.
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 von Mises likelihood estimates von Mises likelihood estimates
-  * R Package: circular+  * see http://en.wikipedia.org/wiki/Von_Mises_distribution 
 +  * R Package: CircStats 
 +  * http://finzi.psych.upenn.edu/R/library/CircStats/html/vm.ml.html 
 +  * vm.ml(...) 
 +  * mu (mean), kappa (concentration parameter) is returned.  
 +   
 +  * R Package: circular (more parameters)
   * http://finzi.psych.upenn.edu/R/library/circular/html/mle.vonmises.html   * http://finzi.psych.upenn.edu/R/library/circular/html/mle.vonmises.html
   * function mle.vonmises(...)   * function mle.vonmises(...)
 +  * Maximum likelihood estimate of concentration parameter (kappa) is a good indicator of bias in directionality. 
  
-Maximum likelihood estimate of concentration parameter (kappa) is a good indicator of bias in directionality+Data Plotting 
 +  * R Package: CircStats 
 +  * http://finzi.psych.upenn.edu/R/library/CircStats/html/00Index.html
  
  
documents/101021cbbpractical.txt · Last modified: 2016/05/24 12:46 by 127.0.0.1

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