User Tools

Site Tools


documents:frapmanu

Differences

This shows you the differences between two versions of the page.


Previous revision
documents:frapmanu [2023/05/30 23:51] (current) kota
Line 1: Line 1:
 +====== FRAP analysis manual ======
 +
 +Dropbox link:
 +
 +[[https://www.dropbox.com/s/du3vqh5v9yjitny/FRAPmanual7bdir.pdf?dl=1|FRAPmanual7bdir.pdf]] 
 +
 +
 +===== FAQ =====
 +
 +<blockquote>
 + My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value makes sense in my experiments, am I allowed just simply to ignore the large tau value?
 +</blockquote>
 +
 +The double exponential fits almost any FRAP curve (even for diffusion-only recovery). This causes problems in the interpretation of data: it could be that kinetics being is nothing to do with the double exponential. 
 +
 +If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences. 
 +
 +If such a condition is not met, forget about the model behind the recovery. Then all you could do is compare the half-max time (1/2 tau). This is rather qualitative but still valid and scientific.
  

Donate Powered by PHP Valid HTML5 Valid CSS Driven by DokuWiki