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--- downloads:bleach_corrector [2012/07/02 10:39] – kota
+++ downloads:bleach_corrector [2021/10/08 08:10] (current) – [Author] kota
@@ Line 1: / Line 1: @@
 ====== Bleach Corrector ======
+{{ :downloads:bleach_correction.mp4 |}}
 ===== Author =====
 Kota Miura\\
-Centre for Molecular and Cellular Imaging (CMCI)\\
+Bioimage Analysis & Research (BIAR)\\
-EMBL Heidelberg\\
+Heidelberg\\
-miura at embl dot de\\
-Tel: +49 6221 387 404
+Video: Christophe Leterrier(@chrislet) [[https://twitter.com/christlet/status/1445658436276867081|See here for the twitter presenting this video]]
+===== Citing the Plugin =====
+  *  Paper: Miura K. Bleach correction ImageJ plugin for compensating the photobleaching of time-lapse sequences [version 1]. F1000Research 2020, 9:1494 [[https://doi.org/10.12688/f1000research.27171.1]]
+  *  Code: Kota Miura et al. (2014). ImageJ Plugin CorrectBleach V2.0.2. Zenodo. [[http://dx.doi.org/10.5281/zenodo.30769|10.5281/zenodo.30769]]
 ===== History =====
@@ Line 14: / Line 21: @@
   * 12-04-16 Compiled with JRE 1.6.0_20
 ==== recent changes ====
-{{rss>https://github.com/cmci/CorrectBleach_/commits/master.atom 10 date 48h}}
+{{rss>https://github.com/fiji/CorrectBleach/commits/master.atom 10 date 48h}}
 ===== Source =====
@@ Line 56: / Line 63: @@
 ==== Q & A ====
-<quote>
+<blockquote>
 One of our users is making timelapse experiments to track a GFP marker in cell cultures. GFP signal is very dim and background is quite strong (so SNR very poor). Over the time, background intensity decreases while specific signal keeps more or less the same so it becomes gradually more visible. He really expects the GFP to increase over the time, and he would like to quantify this increase in GFP signal over time. To compensate background bleaching he is using your bleach_corrector plugin in FIJI. He obtains the best visualization of what he expects with the Histogram Matching Method. The thing is that, as you mention in your blog's entry (http://cmci.embl.de/downloads/bleach_corrector,  http://cmci.embl.de/blogtng/2010-05-06/bleach_correction_2 ), with this method you cannot quantify intensities.
@@ Line 67: / Line 74: @@
 Xavier Sanjuan (ALMU, Parc de Recerca Biomèdica de Barcelona), \\
 on behalf of Diego Barcena (Mark Isalan group, CRG, Barcelona)
-</quote>
+</blockquote>
 \\
 \\