downloads:frap_analysis
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~~NOTOC~~ | ~~NOTOC~~ | ||
- | [{{ http:// | + | [{{ http:// |
====== FrapCalc ====== | ====== FrapCalc ====== | ||
- | Analyze FRAP curves and get parameter by curve fitting ([[downloads:frapDocumentation|documentation.]]). | + | |
+ | **Please jump to [[https:// | ||
+ | |||
+ | <color WhiteSmoke> | ||
===== Author ===== | ===== Author ===== | ||
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Tel: +49 6221 387 404 | Tel: +49 6221 387 404 | ||
===== Questions & Comments ===== | ===== Questions & Comments ===== | ||
- | + | ? | |
Please post in the commenting section below at the bottom of this page | Please post in the commenting section below at the bottom of this page | ||
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===== Installation ===== | ===== Installation ===== | ||
- | Download [[http:// | + | Download [[http:// |
===== Description ===== | ===== Description ===== | ||
Analyze FRAP curves to derive recovery parameters. | Analyze FRAP curves to derive recovery parameters. | ||
- | **More details are in the [[http:// | + | /* |
+ | **More details are in the [[http:// | ||
+ | */ | ||
+ | **More details are in the [[http:// | ||
+ | |||
+ | ===== Features ===== | ||
* Import FRAP measurement tab-delimited text files of Zeiss data format (6 different data structures)\\ Leica data format (7 different data structures) - Three different ways of normalizing FRAP curve - Curve fitting by single and double exponential formula and 2 diffusion formula. | * Import FRAP measurement tab-delimited text files of Zeiss data format (6 different data structures)\\ Leica data format (7 different data structures) - Three different ways of normalizing FRAP curve - Curve fitting by single and double exponential formula and 2 diffusion formula. | ||
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* Graphing of the fitted curve and estimation curve without acquisition bleaching. | * Graphing of the fitted curve and estimation curve without acquisition bleaching. | ||
* Batch Fitting | * Batch Fitting | ||
+ | |||
+ | ===== Papers + Misc ===== | ||
+ | |||
+ | ==== Papers using this script ==== | ||
+ | * Runz, H. et al., 2006. Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G. The EMBO journal, 25(13), p.2953-65. [[http:// | ||
+ | * Atlason, P.T. et al., 2007. N-Methyl-D-aspartate (NMDA) receptor subunit NR1 forms the substrate for oligomeric assembly of the NMDA receptor. The Journal of biological chemistry, 282(35), p.25299-307. [[http:// | ||
+ | * Pooler, A.M. & McIlhinney, R.A.J., 2007. Lateral diffusion of the GABAB receptor is regulated by the GABAB2 C terminus. The Journal of biological chemistry, 282(35), p.25349-56. [[http:// | ||
+ | * Hoppa, M.B. et al., 2009. Chronic palmitate exposure inhibits insulin secretion by dissociation of Ca(2+) channels from secretory granules. Cell metabolism, 10(6), p.455-65. [[http:// | ||
+ | * Treeck, M. et al., 2009. Functional analysis of the leading malaria vaccine candidate AMA-1 reveals an essential role for the cytoplasmic domain in the invasion process. PLoS pathogens, 5(3), p.e1000322. [[http:// | ||
+ | * Bancaud, A., Huet, S., Rabut, G., Ellenberg, J., 2010. Fluorescence perturbation techniques to study mobility and molecular dynamics of proteins in live cells: FRAP, photoactivation, | ||
+ | * Bosch-Presegué, | ||
+ | * Caillier, B. et al., 2011. QRS widening and QT prolongation under bupropion: a unique cardiac electrophysiological profile. Fundamental & clinical pharmacology. [[http:// | ||
+ | * Díaz, A. et al., 2011. Processivity and subcellular localization of glycogen synthase depend on a non-catalytic high affinity glycogen-binding site. The Journal of biological chemistry, 286(21), p.18505-14. [[http:// | ||
+ | * Coutinho-Budd J, Ghukasyan V, Zylka MJ, Polleux F. The F-BAR domains from srGAP1, srGAP2 and srGAP3 regulate membrane deformation differently. Journal of cell science. 2012;125(Pt 14): | ||
+ | ... searches were done by google hits, so if YOURS is missing please tell me! | ||
+ | ==== Web Back Links ==== | ||
+ | |||
+ | * [[http:// | ||
+ | * [[http:// | ||
+ | * MatLab-migrated version of FRAPCalc. | ||
+ | * [[http:// | ||
+ | * [[http:// | ||
+ | * [[http:// | ||
+ | * Dissertation using FRAPCalc | ||
+ | * [[http:// | ||
+ | |||
+ | |||
===== History ===== | ===== History ===== | ||
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* 07-8-27 ver 9a | * 07-8-27 ver 9a | ||
* Added Leica new file format importing (csv format) | * Added Leica new file format importing (csv format) | ||
- | * Made K_SoumpasisHalfLife2(fitwave, | + | * Made K_SoumpasisHalfLife2(fitwave, |
* 07-10-30 ver9b | * 07-10-30 ver9b | ||
* For details, read the [[http:// | * For details, read the [[http:// | ||
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* 09-10-26 ver9h | * 09-10-26 ver9h | ||
* Fitted parameters appear in plot legend even when mobile fraction >1.0. (C. Tischer' | * Fitted parameters appear in plot legend even when mobile fraction >1.0. (C. Tischer' | ||
- | * Added documentation for gammaQ=1 cases. See -> 2.7. [[http:// | + | * Added documentation for gammaQ=1 cases. See -> 2.7. [[http:// |
* 10-03-03 ver9j | * 10-03-03 ver9j | ||
* Batch Processing funciton with averaging function (request from N. Goldsimth). See additional explanation in the [[documents: | * Batch Processing funciton with averaging function (request from N. Goldsimth). See additional explanation in the [[documents: | ||
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* Fitting of averaged curve is done with actual standard deviation | * Fitting of averaged curve is done with actual standard deviation | ||
* BatchFitting() was added to the last line of BatchAverageAndFit() | * BatchFitting() was added to the last line of BatchAverageAndFit() | ||
+ | |||
+ | </ |
downloads/frap_analysis.txt · Last modified: 2017/05/11 15:28 by kota