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downloads:frap_analysis [2010/03/10 09:02] kotadownloads:frap_analysis [2017/05/11 15:28] (current) kota
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 [{{ http://cmci.embl.de/dls/FRAPmanual_files/image069.jpg}}] [{{ http://cmci.embl.de/dls/FRAPmanual_files/image069.jpg}}]
 ====== FrapCalc ====== ====== FrapCalc ======
-Analyze FRAP curves and get parameter by curve fitting ([[downloads:frapDocumentation|documentation.]]). + 
 +**Please jump to [[https://cmci-embl.gitlab.io/docs/analysis/frapcalc/|the new page!]]** 
 + 
 +/** 
 + 
 +Analyze FRAP curves and get parameter by curve fitting ([[http://cmci.embl.de/documents/frapmanu|documentation.]]). Lecture notes are also available ([[http://cmci.embl.de/documents/100420frapinternal | Lecture Notes (Apr. 2010)]]) 
  
 ===== Author ===== ===== Author =====
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 ===== Installation ===== ===== Installation =====
  
-Download [[http://cmci.embl.de/dls/K_FRAPcalcV9j.ipf|K_FRAPcalcV9j.ipf]] to a place where you can access easily. Open the file in IgorPro and compile.+Download [[http://cmci.embl.de/dls/K_FRAPcalcV9k.ipf|K_FRAPcalcV9k.ipf]] to a place where you can access easily (you might need to right click or ctrl-click and then save it to your local machine). Open the file in IgorPro and compile. 
 ===== Description ===== ===== Description =====
  
 Analyze FRAP curves to derive recovery parameters. Analyze FRAP curves to derive recovery parameters.
-/*  
-**More details are in the [[http://cmci.embl.de/dls/FRAPmanual.htm|documentation.]]* 
-*/ 
-**More details are in the [[http://cmci.embl.de/documents/frapmanu|documentation.]]* 
  
  ===== Features =====  ===== Features =====
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   * Graphing of the fitted curve and estimation curve without acquisition bleaching.   * Graphing of the fitted curve and estimation curve without acquisition bleaching.
   * Batch Fitting   * Batch Fitting
 +
 +===== Papers + Misc =====
 +
 +==== Papers using this script ====
 +  * Runz, H. et al., 2006. Sterols regulate ER-export dynamics of secretory cargo protein ts-O45-G. The EMBO journal, 25(13), p.2953-65. [[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1500972&tool=pmcentrez&rendertype=abstract|Accessed February 7, 2012]].
 +  * Atlason, P.T. et al., 2007. N-Methyl-D-aspartate (NMDA) receptor subunit NR1 forms the substrate for oligomeric assembly of the NMDA receptor. The Journal of biological chemistry, 282(35), p.25299-307. [[http://www.ncbi.nlm.nih.gov/pubmed/17606616|Accessed February 7, 2012]].
 +  * Pooler, A.M. & McIlhinney, R.A.J., 2007. Lateral diffusion of the GABAB receptor is regulated by the GABAB2 C terminus. The Journal of biological chemistry, 282(35), p.25349-56. [[http://www.ncbi.nlm.nih.gov/pubmed/17597070|Accessed February 7, 2012]].
 +  * Hoppa, M.B. et al., 2009. Chronic palmitate exposure inhibits insulin secretion by dissociation of Ca(2+) channels from secretory granules. Cell metabolism, 10(6), p.455-65. [[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2814048&tool=pmcentrez&rendertype=abstract|Accessed February 7, 2012]].
 +  * Treeck, M. et al., 2009. Functional analysis of the leading malaria vaccine candidate AMA-1 reveals an essential role for the cytoplasmic domain in the invasion process. PLoS pathogens, 5(3), p.e1000322. [[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2654807&tool=pmcentrez&rendertype=abstract|Accessed February 7, 2012]].
 +  * Bancaud, A., Huet, S., Rabut, G., Ellenberg, J., 2010. Fluorescence perturbation techniques to study mobility and molecular dynamics of proteins in live cells: FRAP, photoactivation, photoconversion, and FLIP. Cold Spring Harb. Protoc. 2010, pdb.top90.
 +  * Bosch-Presegué, L. et al., 2011. Stabilization of Suv39H1 by SirT1 is part of oxidative stress response and ensures genome protection. Molecular cell, 42(2), p.210-23. [[http://www.ncbi.nlm.nih.gov/pubmed/21504832|Accessed January 30, 2012]].
 +  * Caillier, B. et al., 2011. QRS widening and QT prolongation under bupropion: a unique cardiac electrophysiological profile. Fundamental & clinical pharmacology. [[http://www.ncbi.nlm.nih.gov/pubmed/21623902|Accessed February 7, 2012]].
 +  * Díaz, A. et al., 2011. Processivity and subcellular localization of glycogen synthase depend on a non-catalytic high affinity glycogen-binding site. The Journal of biological chemistry, 286(21), p.18505-14. [[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3099667&tool=pmcentrez&rendertype=abstract|Accessed February 7, 2012]].
 +  * Coutinho-Budd J, Ghukasyan V, Zylka MJ, Polleux F. The F-BAR domains from srGAP1, srGAP2 and srGAP3 regulate membrane deformation differently. Journal of cell science. 2012;125(Pt 14):3390–401. [[ http://www.ncbi.nlm.nih.gov/pubmed/22467852|Accessed October 10, 2012]].
 +... searches were done by google hits, so if YOURS is missing please tell me!
 +==== Web Back Links ====
 +
 +  * [[http://www.leica-microsystems.com/science-lab/fluorescence-recovery-after-photobleaching-frap-its-offspring/|Leica Frap Page]]
 +  * [[http://actinsim.uni.lu/eng/Downloads|Univ. of Luxemburg, ActSim download page]]
 +    * MatLab-migrated version of FRAPCalc.
 +  * [[http://adm.irbbarcelona.org/f-r-a-p|ADM, IRB Barcelona]]
 +  * [[http://www.igorexchange.com/node/2330|IgorExchange Forum]]
 +  * [[http://www.ncbi.nlm.nih.gov/pubmed?term=20063225%20| ... citing my lecture in a paper]]
 +  * Dissertation using FRAPCalc
 +    * [[http://hss.ulb.uni-bonn.de/2009/1863/1863.pdf|Luciana Renna, Univ. Bonn]]
 + 
 +
 ===== History ===== ===== History =====
  
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     * Fitting of averaged curve is done with actual standard deviation      * Fitting of averaged curve is done with actual standard deviation 
     * BatchFitting() was added to the last line of BatchAverageAndFit()      * BatchFitting() was added to the last line of BatchAverageAndFit() 
 +
 +
 +*/
downloads/frap_analysis.1268211745.txt.gz · Last modified: 2016/05/24 12:46 (external edit)

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