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FrapCalc

Analyze FRAP curves and get parameter by curve fitting (documentation.).

Author

Kota Miura
Centre for Molecular and Cellular Imaging (CMCI)
EMBL Heidelberg
miura at embl dot de
Tel: +49 6221 387 404

Questions & Comments

? Please post in the commenting section below at the bottom of this page

Source

Released under the GNU General Public License.

Requires

IgorPro ver 5 or higher (IgorPro, Wavemetrics).

Installation

Download K_FRAPcalcV9j.ipf to a place where you can access easily. Open the file in IgorPro and compile.

Description

Analyze FRAP curves to derive recovery parameters. More details are in the documentation.

Features

  • Import FRAP measurement tab-delimited text files of Zeiss data format (6 different data structures)
    Leica data format (7 different data structures) - Three different ways of normalizing FRAP curve - Curve fitting by single and double exponential formula and 2 diffusion formula.
  • Correction for the acquisition bleaching.
  • Output of half-max, diffusion coefficient, mobile/immobile fractions.
  • Weighting for the fitting.
  • Evaluation of goodness of fit by chi-square and gamma function.
  • Graphing of the fitted curve and estimation curve without acquisition bleaching.
  • Batch Fitting

History

  • 05-9-9
    • 1st version
  • 07-3-28
    • 9th version
  • 07-8-27 ver 9a
    • Added Leica new file format importing (csv format)
    • Made K_SoumpasisHalfLife2(fitwave,amplitude) for calculateing Half max. Previous one had problem when point 1 is larger than 0.5 ? Changes for the texts in the graph (Half max value added to diffusion fittings) see more details in the code.
  • 07-10-30 ver9b
    • For details, read the blog
  • 08-03-25 ver 9c
  • 08-07-24 ver 9e
    • Added two data set types for importing. Olympus .cvs file is now possible.
  • 08-10-07 ver9f
    • Added Excel file importing funciton (Four column, with Time-Frap-All Cell- Base, with the first line containg column name) and tab-delimited text file.
  • 08/12/11 ver9g
    • Replaced fitting function for double-exponential fitting, follwoing the recommendation of wavemetrics.
    • Half-time estimation for double exponential fitting had a bug, when data range is below the half-max intensity. This is now done by extrapolation.
  • 09-10-26 ver9h
  • 10-03-03 ver9j
    • Batch Processing funciton with averaging function (request from N. Goldsimth). See additional explanation in the documentation page
    • K_FrapCalcCore() was modified so that averaged curve could be fitted as well. Since averaged curve does not have original normalizaton process is not required but then several parameters which are calculated during normalization will be missing. For this, flag “G_doAveraged” was added
    • getAverageGApRatio(Gfilelist): Gap Ratio is calculated by averaging individual
    • Fitting of averaged curve is done with actual standard deviation
    • BatchFitting() was added to the last line of BatchAverageAndFit()
downloads/frap_analysis.1272012032.txt.gz · Last modified: 2016/05/24 12:46 (external edit)

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