Differences

This shows you the differences between two versions of the page.

--- downloads:kymoquant [2010/03/11 09:18] – kota
+++ downloads:kymoquant [2012/07/11 15:10] – external edit 127.0.0.1
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-~~NOTOC~~
-[{{ http://cmci.embl.de/dls/Kymoquant/overview_kymoquant.jpg?200|Measure Movement directly from kymograph}}]
-====== Kymoquant ======
-Quantitative analysis of ambiguous patterns in kymograph
-===== Author =====
-Kota Miura\\
-Centre for Molecular and Cellular Imaging (CMCI)\\
-EMBL Heidelberg\\
-miura at embl dot de\\
-Tel: +49 6221 387 404
-===== History =====
-  * 10-03-03 ? first version released
-===== Source =====
-Released under the GNU General Public License.
-===== Requires =====
-ImageJ ver 1.43o or higher ([[http://rsb.info.nih.gov/ij/upgrade/|ImageJ, upgrade page]]).
-===== Installation =====
-Download [[http://cmci.embl.de/dls/K_kymoquant.ijm|K_kymoquant.ijm]] to a place where you can access easily. Install the macro by [Plugins->Macros->Install...].
-===== Description =====
-Measures orientation of kymograph by estimating the direction of image texture. Outputs movement velocity in pixels / frame.
-(more explanation on measurement principle is planned here)
-**Tip**: When kymograph traces are steep, analysis results become unstable since calculation of direction involves tanθ. Be sure to capture original sequence with high time resolution if possible.
-===== Work Flow =====
-Kymograph should be prepared by your self either by manually using stack reslicing function in ImageJ, or ImageJ Kymograph plugin.
-If you want to measure full frame of the kymograph, simply apply **[Plugins -> Macros -> Measure Velocity from Kymograph: Full Frame]**.
-If you want to measure only part of the kymograph, then select a area by rectangular ROI tool and then **[Plugins -> Macros -> Measure Velocity from Kymograph: ROI]**.
-**[Plugins -> Macros -> Scan Kymograph and Measure - Automatic Multiple Measurements]** will automatically divide the kymograph into segments and sequentially measure the kymograph along the time line.
-[{{ http://cmci.embl.de/dls/Kymoquant/overview_kymoquant.jpg }}]