downloads:kymoquant
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- | ~~NOTOC~~ | ||
- | [{{ http:// | ||
- | ====== Kymoquant ====== | ||
- | Quantitative analysis of ambiguous patterns in kymograph | ||
- | ===== Author ===== | ||
- | |||
- | Kota Miura\\ | ||
- | Centre for Molecular and Cellular Imaging (CMCI)\\ | ||
- | EMBL Heidelberg\\ | ||
- | miura at embl dot de\\ | ||
- | Tel: +49 6221 387 404 | ||
- | |||
- | ===== History ===== | ||
- | |||
- | * 10-03-03 ? first version released | ||
- | |||
- | ===== Source ===== | ||
- | |||
- | Released under the GNU General Public License. | ||
- | ===== Requires ===== | ||
- | |||
- | ImageJ ver 1.43o or higher ([[http:// | ||
- | ===== Installation ===== | ||
- | |||
- | Download [[http:// | ||
- | ===== Description ===== | ||
- | |||
- | Measures orientation of kymograph by estimating the direction of image texture. Outputs movement velocity in pixels / frame. | ||
- | |||
- | (more explanation on measurement principle is planned here) | ||
- | |||
- | **Tip**: When kymograph traces are steep, analysis results become unstable since calculation of direction involves tanθ. Be sure to capture original sequence with high time resolution if possible. | ||
- | |||
- | ===== Work Flow ===== | ||
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- | Kymograph should be prepared by your self either by manually using stack reslicing function in ImageJ, or ImageJ Kymograph plugin. | ||
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- | If you want to measure full frame of the kymograph, simply apply **[Plugins -> Macros -> Measure Velocity from Kymograph: Full Frame]**. | ||
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- | If you want to measure only part of the kymograph, then select a area by rectangular ROI tool and then **[Plugins -> Macros -> Measure Velocity from Kymograph: ROI]**. | ||
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- | **[Plugins -> Macros -> Scan Kymograph and Measure - Automatic Multiple Measurements]** will automatically divide the kymograph into segments and sequentially measure the kymograph along the time line. | ||
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- | [{{ http:// |
downloads/kymoquant.txt · Last modified: 2016/05/24 12:46 by 127.0.0.1