downloads:kymoquant
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+ | ~~NOTOC~~ | ||
+ | [{{ http:// | ||
+ | ====== Kymoquant ====== | ||
+ | Quantitative analysis of ambiguous patterns in kymograph | ||
+ | ===== Author ===== | ||
+ | |||
+ | Kota Miura\\ | ||
+ | Centre for Molecular and Cellular Imaging (CMCI)\\ | ||
+ | EMBL Heidelberg\\ | ||
+ | miura at embl dot de\\ | ||
+ | Tel: +49 6221 387 404 | ||
+ | |||
+ | ===== History ===== | ||
+ | |||
+ | * 10-03-03 ? first version released | ||
+ | |||
+ | ===== Source ===== | ||
+ | |||
+ | Released under the GNU General Public License. | ||
+ | ===== Requires ===== | ||
+ | |||
+ | ImageJ ver 1.43o or higher ([[http:// | ||
+ | ===== Installation ===== | ||
+ | |||
+ | Download [[https:// | ||
+ | ===== Description ===== | ||
+ | |||
+ | Measures orientation of kymograph by estimating the direction of image texture. Outputs movement velocity in pixels / frame. | ||
+ | |||
+ | (more explanation on measurement principle is planned here) | ||
+ | |||
+ | **Tip**: When kymograph traces are steep, analysis results become unstable since calculation of direction involves tanθ. Be sure to capture original sequence with high time resolution if possible. | ||
+ | |||
+ | ===== Work Flow ===== | ||
+ | |||
+ | Kymograph should be prepared by your self either by manually using stack reslicing function in ImageJ, or ImageJ Kymograph plugin. | ||
+ | |||
+ | If you want to measure full frame of the kymograph, simply apply **[Plugins -> Macros -> Measure Velocity from Kymograph: Full Frame]**. | ||
+ | |||
+ | If you want to measure only part of the kymograph, then select a area by rectangular ROI tool and then **[Plugins -> Macros -> Measure Velocity from Kymograph: ROI]**. | ||
+ | |||
+ | **[Plugins -> Macros -> Scan Kymograph and Measure - Automatic Multiple Measurements]** will automatically divide the kymograph into segments and sequentially measure the kymograph along the time line. | ||
+ | |||
+ | [{{ http:// |