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downloads:pefileto3dstacks [2010/03/03 15:45] kota created |
downloads:pefileto3dstacks [2016/05/24 12:46] |
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- | ~~NOTOC~~ | ||
- | ====== File Converter - Perkin Elmer Tiff Series to 3D stacks ====== | ||
- | ==== Author ==== | ||
- | |||
- | Kota Miura\\ | ||
- | Centre for Molecular and Cellular Imaging (CMCI)\\ | ||
- | EMBL Heidelberg\\ | ||
- | miura at embl dot de Tel: +49 6221 387 404 \\ | ||
- | |||
- | ==== History ==== | ||
- | |||
- | 2007/6/5: 1st version\\ \\ | ||
- | ==== Source ==== | ||
- | |||
- | Released under the GNU General Public License. | ||
- | ==== Requires ==== | ||
- | |||
- | ImageJ ver 1.37j or higher ([[http:// | ||
- | ==== Installation ==== | ||
- | |||
- | Download [[http:// | ||
- | |||
- | ==== Description ==== | ||
- | |||
- | Converts xyzt time series (single tiff series) generated by[[http:// | ||
- | Z000_c1_T0000.tif | ||
- | Z000_c1_T0001.tif | ||
- | ... | ||
- | Z%%***_%%c1_T%%****%%.tif | ||
- | The macro produces Z-stack for each time points (metamorph format). For example, if there were 30 Z slices and 100 time points, original tiff-series would have 3000 single XY files. This macro converts these 3000 files to 100 tiff-stacks, | ||
- | In addition, the macro generates a stack named " | ||
- | ==== Work Flow ==== | ||
- | |||
- | **1. ** After installation, | ||
- | **2.** Do **[Plugins -> Macros -> do Processing]**. In the first dialog window, input the number of z-slices. In the second dialog, input the number of time points. Be Careful: since the frame number start from 0, the last frame number + 1 is the total number of frames in z or t. For example, if the last time point is " | ||
- | 3. In [[http:// | ||
- | 4. In [[http:// | ||
- | \\ \\ | ||
- | **Acknowledgement: |