Monday, April 24, 2006

Answering to a question from Israel

how to align lots of movies wich has a temperature dependend x,y shift. Do you know any good plugin or another reasonable program to define a static point inside the images ant realign the entire stack to the coordinates of the particle inside the first image?


try using either

http://bigwww.epfl.ch/thevenaz/turboreg/

or

http://bigwww.epfl.ch/thevenaz/stackreg/

I personally have not used it, but I know a guy in EMBL using TurboReg for aligning cell images.

Thursday, April 20, 2006

Multicellular Movements

single Cell migration within multicellualr structure (tissue) contains
(1) passive movement
(2) active movement

if the cells are migrating as a group, these two types of motive force is difficult to distinguish. To Know how a cell withn a group is moving in the contect of the movement of the whole group, subtraction of the movement of the group form individual cell movement is one way.

--> made Igor procedures for this.
K_SubtractBackMoveTrackCore(Prefix,startCell,EndCell)
DisplayBackSubtractedTrack(Prefix,startCell,EndCell)

both are in K_analyzemanualTracked.ipf

Friday, April 07, 2006

codec

Quick time codec comparsion
http://mac.sillydog.org/qt/compare.php