====== Assignments ====== ===== module 1 and 2 ===== ==== 1. Quantum dot counting (planned, but omitted)==== sample image: quantumdots.tif design a protocol for segmenting quantum dot and write a macro that counts the number of quantum dots in the image. ==== 2. Modify dot movement macro as you like. ==== (Brownian, Cycloid, Sinusoidal …) Save your macro in the data server. Please do not forget to include your names in the macro file name. **Answers** * XY movement * see https://gist.github.com/miura/24a6d91c7358454cb444 * Random Walk * see https://gist.github.com/miura/3e1b8bcd73ea864b46ee * The function used in this macro could be tested with the following code * https://gist.github.com/miura/5592101 ===== module 4 ===== ==== Relative Movement ==== Go to section 4.4.7 and do the exercise 4.4.7-1. ==== PIV based anaysis (omitted) ==== Try install and using the following plugin: https://sites.google.com/site/qingzongtseng/piv Get a vector field data (you need only two frames for this), save the results as a csv file and then analyze the directioanlity in R.