Table of Contents

Assignments
- module 1 and 2
  - 1. Quantum dot counting (planned, but omitted)
  - 2. Modify dot movement macro as you like.
- module 4
  - Relative Movement
  - PIV based anaysis (omitted)

Assignments

module 1 and 2

1. Quantum dot counting (planned, but omitted)

sample image: quantumdots.tif

design a protocol for segmenting quantum dot and write a macro that counts the number of quantum dots in the image.

2. Modify dot movement macro as you like.

(Brownian, Cycloid, Sinusoidal …)

Save your macro in the data server. Please do not forget to include your names in the macro file name.

Answers

XY movement
- see https://gist.github.com/miura/24a6d91c7358454cb444
Random Walk
- see https://gist.github.com/miura/3e1b8bcd73ea864b46ee
- The function used in this macro could be tested with the following code
  - https://gist.github.com/miura/5592101

module 4

Relative Movement

Go to section 4.4.7 and do the exercise 4.4.7-1.

PIV based anaysis (omitted)

Try install and using the following plugin: https://sites.google.com/site/qingzongtseng/piv

Get a vector field data (you need only two frames for this), save the results as a csv file and then analyze the directioanlity in R.