downloads:bleach_corrector
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downloads:bleach_corrector [2012/07/02 10:39] – kota | downloads:bleach_corrector [2021/10/08 08:10] (current) – [Author] kota | ||
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====== Bleach Corrector ====== | ====== Bleach Corrector ====== | ||
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+ | {{ : | ||
===== Author ===== | ===== Author ===== | ||
Kota Miura\\ | Kota Miura\\ | ||
- | Centre for Molecular and Cellular Imaging | + | Bioimage Analysis & Research |
- | EMBL Heidelberg\\ | + | Heidelberg\\ |
- | miura at embl dot de\\ | + | |
- | Tel: +49 6221 387 404 | + | Video: Christophe Leterrier(@chrislet) [[https:// |
+ | |||
+ | ===== Citing the Plugin ===== | ||
+ | |||
+ | * Paper: Miura K. Bleach correction ImageJ plugin for compensating the photobleaching of time-lapse sequences [version 1]. F1000Research 2020, 9:1494 [[https:// | ||
+ | * Code: Kota Miura et al. (2014). ImageJ Plugin CorrectBleach V2.0.2. Zenodo. [[http:// | ||
===== History ===== | ===== History ===== | ||
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* 12-04-16 Compiled with JRE 1.6.0_20 | * 12-04-16 Compiled with JRE 1.6.0_20 | ||
==== recent changes ==== | ==== recent changes ==== | ||
- | {{rss> | + | {{rss> |
===== Source ===== | ===== Source ===== | ||
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==== Q & A ==== | ==== Q & A ==== | ||
- | <quote> | + | <blockquote> |
One of our users is making timelapse experiments to track a GFP marker in cell cultures. GFP signal is very dim and background is quite strong (so SNR very poor). Over the time, background intensity decreases while specific signal keeps more or less the same so it becomes gradually more visible. He really expects the GFP to increase over the time, and he would like to quantify this increase in GFP signal over time. To compensate background bleaching he is using your bleach_corrector plugin in FIJI. He obtains the best visualization of what he expects with the Histogram Matching Method. The thing is that, as you mention in your blog's entry (http:// | One of our users is making timelapse experiments to track a GFP marker in cell cultures. GFP signal is very dim and background is quite strong (so SNR very poor). Over the time, background intensity decreases while specific signal keeps more or less the same so it becomes gradually more visible. He really expects the GFP to increase over the time, and he would like to quantify this increase in GFP signal over time. To compensate background bleaching he is using your bleach_corrector plugin in FIJI. He obtains the best visualization of what he expects with the Histogram Matching Method. The thing is that, as you mention in your blog's entry (http:// | ||
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Xavier Sanjuan (ALMU, Parc de Recerca Biomèdica de Barcelona), \\ | Xavier Sanjuan (ALMU, Parc de Recerca Biomèdica de Barcelona), \\ | ||
on behalf of Diego Barcena (Mark Isalan group, CRG, Barcelona) | on behalf of Diego Barcena (Mark Isalan group, CRG, Barcelona) | ||
- | </quote> | + | </blockquote> |
\\ | \\ | ||
\\ | \\ |
downloads/bleach_corrector.1341225547.txt.gz · Last modified: 2016/05/24 12:46 (external edit)