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--- blogtng:2010-05-19:quickpalm [2010/05/19 13:36] – created kota
+++ blogtng:2010-05-19:quickpalm [2020/11/26 09:11] (current) – external edit 127.0.0.1
@@ Line 5: / Line 5: @@
 doi:10.1038/nmeth0510-339
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+<blockquote>
 PALM and STORM reconstruction algorithms usually rely on 'fitting' Gaussian kernels to detected diffraction-limited spots. Although they permit high-accuracy localizations, these iterative methods can require up to several hours of processing time. We have developed a high-speed reconstruction algorithm that uses the classical Högbom 'CLEAN' method8 for spot finding, followed by a modified center of mass algorithm to compute the spot position and parameters defining spot shape along the horizontal axes.
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+</blockquote>
 I have not yet tried this, but seems to be very usable also for simple cases that needs to find spots. Plugin could be downloaded from
 [[http://code.google.com/p/quickpalm/]]