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Timeline of @cmci_

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RT @ChanZuckerberg: The microscope changed the world by allowing us to view cells and treat diseases. Today we announced funding for 17 Ima…
About 18 hours, 17 mins ago by: Kota Miura (@cmci_)

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RT @petebankhead: @Path_Matt @DrMarkOng @Dr_DR_Cells @tralimiguel QuPath & ImageJ can be used together https://t.co/zV1tOlULqy I use ImageJ…
About 19 hours, 44 mins ago by: Kota Miura (@cmci_)

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should read "Paris building" not "meeting"
About 19 hours, 44 mins ago by: Kota Miura (@cmci_)

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RT @CORBEL_eu: The CORBEL #webinar series continues on 11 April 2019 15:30 CEST with "A Basic Introduction to #Bioimage Analysis" https://t…
About 1 day, 2 hours ago by: Kota Miura (@cmci_)

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Growing cytoskeleton on the surface of Paris meeting https://t.co/hIwnFmu64T
About 1 day, 6 hours ago by: Kota Miura (@cmci_)

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He’s now sitting with audience because he’s showing a 6 min film !!!! How lazy the guy is...
About 1 day, 6 hours ago by: Kota Miura (@cmci_)
blogtng:2010-05-19:quickpalm

QuickPALM

QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ Nature Methods 7, 339 - 340 (2010) doi:10.1038/nmeth0510-339

PALM and STORM reconstruction algorithms usually rely on 'fitting' Gaussian kernels to detected diffraction-limited spots. Although they permit high-accuracy localizations, these iterative methods can require up to several hours of processing time. We have developed a high-speed reconstruction algorithm that uses the classical Högbom 'CLEAN' method8 for spot finding, followed by a modified center of mass algorithm to compute the spot position and parameters defining spot shape along the horizontal axes.

I have not yet tried this, but seems to be very usable also for simple cases that needs to find spots. Plugin could be downloaded from

http://code.google.com/p/quickpalm/

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blogtng/2010-05-19/quickpalm.txt · Last modified: 2016/05/24 05:46 (external edit)