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Timeline of @cmci_

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A Job position, KNIME! https://t.co/GmCRWDp0oH
About 2 days, 21 hours ago by: Kota Miura (@cmci_)

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RT @stelfrich: The amazing Anna Klemm from @scilifelab's BioImage Informatics Facility teaching students how to use ImageJ macros to automa…
About 2 days, 22 hours ago by: Kota Miura (@cmci_)

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RT @AssafZaritsky: Mutex Watershed: watershed + long-range repulsions --> accurate segmentation without setting parameters! Performance ass…
About 4 days, 3 hours ago by: Kota Miura (@cmci_)

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RT @Irate_Physicist: A very nice review of cell nuclear mechanics https://t.co/JLQgHLi49U
About 1 week, 1 day ago by: Kota Miura (@cmci_)

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RT @HenriquesLab: @OptNanoscopy, as promised here's the presentation I've done for #SMolBac "Democratising live-cell super-resolution micro…
About 1 week, 2 days ago by: Kota Miura (@cmci_)

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RT @embl: Today is the final day of this #EIPOD call. Don't miss the deadline if you have not completed your application yet! https://t.co/
About 1 week, 2 days ago by: Kota Miura (@cmci_)
blogtng:2010-05-19:quickpalm

QuickPALM

QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ Nature Methods 7, 339 - 340 (2010) doi:10.1038/nmeth0510-339

PALM and STORM reconstruction algorithms usually rely on 'fitting' Gaussian kernels to detected diffraction-limited spots. Although they permit high-accuracy localizations, these iterative methods can require up to several hours of processing time. We have developed a high-speed reconstruction algorithm that uses the classical Högbom 'CLEAN' method8 for spot finding, followed by a modified center of mass algorithm to compute the spot position and parameters defining spot shape along the horizontal axes.

I have not yet tried this, but seems to be very usable also for simple cases that needs to find spots. Plugin could be downloaded from

http://code.google.com/p/quickpalm/

Comments



134 -2 = 
blogtng/2010-05-19/quickpalm.txt · Last modified: 2016/05/24 05:46 (external edit)