documents:frapmanu
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- | {{url> | + | ====== FRAP analysis manual ====== |
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+ | Dropbox link: | ||
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+ | [[https://www.dropbox.com/s/du3vqh5v9yjitny/ | ||
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+ | ===== FAQ ===== | ||
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+ | < | ||
+ | My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value makes sense in my experiments, | ||
+ | </ | ||
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+ | The double exponential fits almost any FRAP curve (even for diffusion-only recovery). This causes problems in the interpretation of data: it could be that kinetics being is nothing to do with the double exponential. | ||
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+ | If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences. | ||
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+ | If such a condition is not met, forget about the model behind the recovery. Then all you could do is compare the half-max time (1/2 tau). This is rather qualitative but still valid and scientific. |
documents/frapmanu.1267630803.txt.gz · Last modified: 2016/05/24 12:46 (external edit)