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EMBL BioImage Data Analysis

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Timeline of @cmci_

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Great results and I will try to keep my house with higher RH in winter but how were they tracked? In methods, it sa… https://t.co/ECVKz0diPN
About 23 hours, 33 mins ago by: Kota Miura (@cmci_)

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Pi (1998), The must see movie No.1 for Bioimage Analysts. https://t.co/EhpvAPVCoY
About 1 day, 8 hours ago by: Kota Miura (@cmci_)

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codes, sample images and workflow descriptions are all freely accessible. very very good. https://t.co/BYQCunBWDl
About 2 days, 4 hours ago by: Kota Miura (@cmci_)

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Quote: "an ImageJ macro called AggreCount to identify and measure protein aggregates in cells" #BioImageAnalysis https://t.co/mb8EX2GcFg
About 2 days, 4 hours ago by: Kota Miura (@cmci_)

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RT @cdossman: A #python package for data handling and evaluation in deep learning-based medical image analysis Github: https://t.co/6cPkDV
About 2 days, 4 hours ago by: Kota Miura (@cmci_)

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@joachimgoedhart Ik hoop dat je snel beter wordt en ons meer magie laat zien!
About 2 days, 4 hours ago by: Kota Miura (@cmci_)
documents:frapmanu

FRAP analysis manual

The document is temporally shown via google drive (which is difficult to view). You could adjust the zooming by yourself, or feel free to download via File menu within the interface.

FAQ

My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value make sense in my experiments, am I allowed just simply to ignore the large tau value?

The double exponential fits to almost any FRAP curve (even for diffusion only recovery). This causes problem in interpretation of data: it could be that kinetics beihing is nothing to do with the double exponential.

If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences.

If such condition is not met, forget about the model behind the recovery. Then all you could do is to compare the half-max time (1/2 tau). This is rather qualitative, but still is valid and scientific.

documents/frapmanu.txt · Last modified: 2016/05/24 12:46 (external edit)