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EMBL BioImage Data Analysis

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RT @EuroBioImaging: Last chance to present your bioimaging analysis work at 4th NEUBIAS conference ๐Ÿ‘‡ https://t.co/D4jfEVfWGo
About 1 day, 8 hours ago by: Kota Miura (@cmci_)

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RT @f_levet: You have until next monday to submit an abstract to the @NEUBIAS_COST Symposium. It's your last chance ! Go go go !
About 1 day, 9 hours ago by: Kota Miura (@cmci_)

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Bioimage Analysis Lunch Heidelberg, Jan 2020 https://t.co/6FcCNzu1QJ
About 1 day, 9 hours ago by: Kota Miura (@cmci_)

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RT @Datascience__: DL@MBL: Deep Learning for Microscopy Image Analysis https://t.co/DT25zxxaRn #DeepLearning
About 2 days, 8 hours ago by: Kota Miura (@cmci_)

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@LauLauThom well done Laurent!
About 2 days, 12 hours ago by: Kota Miura (@cmci_)

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RT @LauLauThom: Another documentation gem for #ImageJ / #Fiji geeks https://t.co/GgC2rRSEYo https://t.co/Gce9E0o8Xt
About 4 days, 6 hours ago by: Kota Miura (@cmci_)
documents:frapmanu

FRAP analysis manual

The document is temporally shown via google drive (which is difficult to view). You could adjust the zooming by yourself, or feel free to download via File menu within the interface.

FAQ

My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value make sense in my experiments, am I allowed just simply to ignore the large tau value?

The double exponential fits to almost any FRAP curve (even for diffusion only recovery). This causes problem in interpretation of data: it could be that kinetics beihing is nothing to do with the double exponential.

If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences.

If such condition is not met, forget about the model behind the recovery. Then all you could do is to compare the half-max time (1/2 tau). This is rather qualitative, but still is valid and scientific.

documents/frapmanu.txt ยท Last modified: 2016/05/24 05:46 (external edit)