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Anna getting ready for her talk @NEUBIAS_COST workshop starting in 3 min. https://t.co/Dl9ZI0NKDs
About 6 hours ago by: Kota Miura (@cmci_)

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RT @MIAP_Freiburg: @MIAP_Freiburg @UniFreiburg will host another basic Fiji @FijiSc/#ImageJ workshop on November 14th-15th 2018 with Kota M…
About 6 hours, 1 min ago by: Kota Miura (@cmci_)

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RT @haesleinhuepf: Let's measure with @laure_plantard and #ImageJ at #NEUBIAS #TS8 🤘 https://t.co/oVpyDWP3HT
About 1 day ago by: Kota Miura (@cmci_)

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RT @haesleinhuepf: Tracking cells on steroids: @jytinevez introducing #Mamut just next door #NEUBIAS #TS9 https://t.co/cKYq55i2JU
About 1 day ago by: Kota Miura (@cmci_)

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RT @haesleinhuepf: Anna Klemm from @scilifelab lifting us to the next level: tracking objects #NEUBIAS #TS8 https://t.co/FSkyLXilAO
About 1 day ago by: Kota Miura (@cmci_)

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RT @dn_mason: Check out our latest @biorxivpreprint (/w @Rosalierrr @ViolaineSee, @raphavisses & Rachel Bearon)! A workflow for imaging, tr…
About 1 day, 2 hours ago by: Kota Miura (@cmci_)
documents:frapmanu

FRAP analysis manual

The document is temporally shown via google drive (which is difficult to view). You could adjust the zooming by yourself, or feel free to download via File menu within the interface.

FAQ

My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value make sense in my experiments, am I allowed just simply to ignore the large tau value?

The double exponential fits to almost any FRAP curve (even for diffusion only recovery). This causes problem in interpretation of data: it could be that kinetics beihing is nothing to do with the double exponential.

If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences.

If such condition is not met, forget about the model behind the recovery. Then all you could do is to compare the half-max time (1/2 tau). This is rather qualitative, but still is valid and scientific.

documents/frapmanu.txt · Last modified: 2016/05/24 05:46 (external edit)