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RT @albertcardona: @cmci_ @LauLauThom In 2004 I wrote the "CLI", a command line interpreter for the ImageJ macro language. Its UI is the fo…
About 21 hours, 52 mins ago by: Kota Miura (@cmci_)

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Another biological world under deep earth https://t.co/J38asi2M48
About 1 day, 12 hours ago by: Kota Miura (@cmci_)

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RT @CDBMicroCore: Great summary of different visualization strategies for time-lapse data -- I need to incorporate this into my quantitativ…
About 1 day, 14 hours ago by: Kota Miura (@cmci_)

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@albertcardona @LauLauThom wow, that will be very useful. By the way, I found a thread today related to interpreter… https://t.co/IBlmlXg4tZ
About 1 day, 18 hours ago by: Kota Miura (@cmci_)

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RT @albertcardona: @cmci_ @LauLauThom Cool! By the way, I've augmented the Script Editor with an "incremental" evaluation mode: via a comma…
About 1 day, 21 hours ago by: Kota Miura (@cmci_)

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@LauLauThom">LauLauThom thanks @LauLauThom">LauLauThom !
About 2 days, 8 hours ago by: Kota Miura (@cmci_)
documents:frapmanu

FRAP analysis manual

The document is temporally shown via google drive (which is difficult to view). You could adjust the zooming by yourself, or feel free to download via File menu within the interface.

FAQ

My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value make sense in my experiments, am I allowed just simply to ignore the large tau value?

The double exponential fits to almost any FRAP curve (even for diffusion only recovery). This causes problem in interpretation of data: it could be that kinetics beihing is nothing to do with the double exponential.

If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences.

If such condition is not met, forget about the model behind the recovery. Then all you could do is to compare the half-max time (1/2 tau). This is rather qualitative, but still is valid and scientific.

documents/frapmanu.txt · Last modified: 2016/05/24 05:46 (external edit)