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The field for bioimage analyst expanding. British museum! https://t.co/KClNvxP948
About 2 days, 5 hours ago by: Kota Miura (@cmci_)

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RT @dgaboriau: Panel session: should I aim to become a bioimage analyst? With Robert Hasse @haesleinhuepf , Kota Miura @cmci_ and Arianne B…
About 3 days, 19 hours ago by: Kota Miura (@cmci_)

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RT @martinjones78: Shoutout by @cmci_ for @clathrin's book https://t.co/x6d2OmfdxR as a great resource, here at #CBIAS2022 in the panel ses…
About 3 days, 19 hours ago by: Kota Miura (@cmci_)

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RT @alex_paynedwyer: The one and only Kota Miura @cmci_ calling out all those dodgy contrast-mismatched figures #ethics in classic humorou…
About 4 days, 3 hours ago by: Kota Miura (@cmci_)

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RT @RekadZeinab: Kicking the last session of #CBIAS2022 with the great master @cmci_ on the challenges of doing image analysis at the inter…
About 4 days, 3 hours ago by: Kota Miura (@cmci_)

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RT @MaKaefer: Fear not: there is something that can be done! Abide by the guidelines or report everything that has been done - whether it i…
About 4 days, 3 hours ago by: Kota Miura (@cmci_)
documents:frapmanu

FRAP analysis manual

The document is temporally shown via google drive (which is difficult to view). You could adjust the zooming by yourself, or feel free to download via File menu within the interface.

FAQ

My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value make sense in my experiments, am I allowed just simply to ignore the large tau value?

The double exponential fits to almost any FRAP curve (even for diffusion only recovery). This causes problem in interpretation of data: it could be that kinetics beihing is nothing to do with the double exponential.

If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences.

If such condition is not met, forget about the model behind the recovery. Then all you could do is to compare the half-max time (1/2 tau). This is rather qualitative, but still is valid and scientific.

documents/frapmanu.txt · Last modified: 2020/11/26 09:11 (external edit)