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EMBL BioImage Data Analysis

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Timeline of @cmci_

cmci_ avatar

... so don't think that you need to submit a full text.
About 3 days, 8 hours ago by: Kota Miura (@cmci_)

cmci_ avatar

The deadline next week for NEUBAIS Gateway article is just for the **PROPOSAL.** you just need to fill and submit… https://t.co/2aTmNDxtvz
About 3 days, 8 hours ago by: Kota Miura (@cmci_)

cmci_ avatar

I really like the article refers to the MoU of NEUBIAS. We made pretty much of efforts in polishing our initial "Ma… https://t.co/E1417kBAAF
About 3 days, 11 hours ago by: Kota Miura (@cmci_)

cmci_ avatar

recommendation to read https://t.co/3BAl3lzcMc
About 3 days, 16 hours ago by: Kota Miura (@cmci_)

cmci_ avatar

RT @florianjug: If you missed the webinar but are interested to have a look, a recording is now available: https://t.co/qVuhexTz3c Thanks a…
About 1 week, 3 days ago by: Kota Miura (@cmci_)

cmci_ avatar

RT @SuperResoluSian: We've collated all the links from our @NEUBIAS_COST webinar on SMLM analysis into a post!⬇️ From here you can navigate…
About 2 weeks ago by: Kota Miura (@cmci_)
documents:frapmanu

FRAP analysis manual

The document is temporally shown via google drive (which is difficult to view). You could adjust the zooming by yourself, or feel free to download via File menu within the interface.

FAQ

My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value make sense in my experiments, am I allowed just simply to ignore the large tau value?

The double exponential fits to almost any FRAP curve (even for diffusion only recovery). This causes problem in interpretation of data: it could be that kinetics beihing is nothing to do with the double exponential.

If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences.

If such condition is not met, forget about the model behind the recovery. Then all you could do is to compare the half-max time (1/2 tau). This is rather qualitative, but still is valid and scientific.

documents/frapmanu.txt · Last modified: 2016/05/24 05:46 (external edit)