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EMBL BioImage Data Analysis

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@EntraCod @COSTprogramme @NEUBIAS_COST Yes everyone indeed. Thanks for the suggestion.
About 1 hour, 12 mins ago by: Kota Miura (@cmci_)

cmci_ avatar

RT @SimonFlyvbjerg: Fresh off Springer's open access press: "Bioimage Data Analysis Workflows" - the book! #BioImageAnalysis https://t.co
About 5 hours, 56 mins ago by: Kota Miura (@cmci_)

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@COSTprogramme @NEUBIAS_COST Thanks to the authors and editors: @perrinepgil Sébastien Tosi Julien Colombelli… https://t.co/GVlRWoqGxr
About 6 hours, 37 mins ago by: Kota Miura (@cmci_)

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Now online, "Bioimage Data Analysis Workflow". https://t.co/stW12drKaS Thanks to the support by @COSTprogramme ,… https://t.co/pgJck70nW6
About 6 hours, 44 mins ago by: Kota Miura (@cmci_)

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RT @Datascience__: Dynamic allocation of computational resources for deep learning-enabled cellular image analysis with Kubernetes https://…
About 3 days, 7 hours ago by: Kota Miura (@cmci_)

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RT @mariana_deniz: Many thanks to the @NEUBIAS_COST instructors and organizers for an exciting and stimulating course on image analysis 😁😁…
About 3 days, 11 hours ago by: Kota Miura (@cmci_)
documents:frapmanu

FRAP analysis manual

The document is temporally shown via google drive (which is difficult to view). You could adjust the zooming by yourself, or feel free to download via File menu within the interface.

FAQ

My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value make sense in my experiments, am I allowed just simply to ignore the large tau value?

The double exponential fits to almost any FRAP curve (even for diffusion only recovery). This causes problem in interpretation of data: it could be that kinetics beihing is nothing to do with the double exponential.

If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences.

If such condition is not met, forget about the model behind the recovery. Then all you could do is to compare the half-max time (1/2 tau). This is rather qualitative, but still is valid and scientific.

documents/frapmanu.txt · Last modified: 2016/05/24 05:46 (external edit)