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--- downloads:spindlefanalyzer [2010/03/05 20:20] – replaced /downloads to /dls kota
+++ downloads:spindlefanalyzer [2020/11/26 09:11] (current) – external edit 127.0.0.1
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-~~DISCUSSION~~
+~~DISCUSSION:closed~~
 ~~NOTOC~~
 [{{ http://cmci.embl.de/dls/SpindleIntensity/Intensity_profile.jpg?250|Measurment of Microtubule Associated Protein Flux}}]
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 ==== Description ====
 The specific purpose of this macro was to follow the intensity dynamics occurring within double labeled microtubule spindle. Intensity changes that are occurring at stable position is easy, but this macro enables measurement of intensity changes that accompanies movement of the bleached/activated position. For the actual application, please refer to the fillowing paper:\\ \\
-<quote>
+<blockquote>
 **Poleward transport of Eg5 by dynein-dynactin in Xenopus laevis egg extract spindles.**\\
 Uteng, M., Hentrich, C., Miura, K., Bieling, P. & Surrey, T.\\ J Cell Biol. 2008 Aug 25;182(4):715-26. Epub 2008 Aug 18.[[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&amp;cmd=Retrieve&amp;list_uids=18710923&amp;dopt=Abstract|PubMed]]
-</quote>\\
+</blockquote>\\
 [{{ http://cmci.embl.de/dls/SpindleIntensity/Rch.jpg?200|Fig.1 Red Channlel/Tubulin}}]
 [{{ http://cmci.embl.de/dls/SpindleIntensity/mask.jpg?200| Fig.2 Tubulin signal converted to mask}}]
 [{{ http://cmci.embl.de/dls/SpindleIntensity/Gch.jpg?200| Fig.3 Green Channlel/MAP}}]
 Using a pair of two-channel image stacks, this macro measures dynamics of integrated intensity profile along x-axis of a channel masked by a mask created using the other channel. For example, we take red channle and green channel  images. In red channle (Fig. 1), tubulin is labled and in the green channel (Fig. 3), certain microtubule-associated protein is labeled. The program first creates a mask (black and white image, Fig. 2) from tubulin R-channel that specifies the area of the spindle. Then this specified area is measured for the integrated intensity profile (projected in y-axis, and measured along x-axis). Same procedure is done for the all frames within the stacks automatically. Profile data will be printed out in the Results window as a table. Another functionincluded in the macro will let the user to plot the multiple profiles in a graph to evaluate the measurements. Results could also be exported and examined using other software such as Excel.?\\ \\
+==== Other papers using this macro ====
+- Fu, J., Bian, M., Xin, G., Deng, Z., Luo, J., Guo, X., Chen, H., Wang, Y., Jiang, Q., Zhang, C., 2015. TPX2 phosphorylation maintains metaphase spindle length by regulating microtubule flux. J. Cell Biol. 210, 373–83.
 ==== Work Flow ====