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--- downloads:kymoquant [2012/07/11 15:10] – external edit 127.0.0.1
+++ downloads:kymoquant [2016/05/24 12:46] (current) – external edit 127.0.0.1
@@ Line 1: / Line 1: @@
+~~NOTOC~~
+[{{ http://cmci.embl.de/dls/Kymoquant/overview_kymoquant.jpg?200|Measure Movement directly from kymograph}}]
+====== Kymoquant ======
+Quantitative analysis of ambiguous patterns in kymograph
+===== Author =====
+Kota Miura\\
+Centre for Molecular and Cellular Imaging (CMCI)\\
+EMBL Heidelberg\\
+miura at embl dot de\\
+Tel: +49 6221 387 404
+===== History =====
+  * 10-03-03 ? first version released
+===== Source =====
+Released under the GNU General Public License.
+===== Requires =====
+ImageJ ver 1.43o or higher ([[http://rsb.info.nih.gov/ij/upgrade/|ImageJ, upgrade page]]).
+===== Installation =====
+Download [[https://raw.github.com/cmci/ijmacros/master/K_kymoquant.ijm|K_kymoquant.ijm]] to a place where you can access easily. Install the macro by [Plugins->Macros->Install...].
+===== Description =====
+Measures orientation of kymograph by estimating the direction of image texture. Outputs movement velocity in pixels / frame.
+(more explanation on measurement principle is planned here)
+**Tip**: When kymograph traces are steep, analysis results become unstable since calculation of direction involves tanθ. Be sure to capture original sequence with high time resolution if possible.
+===== Work Flow =====
+Kymograph should be prepared by your self either by manually using stack reslicing function in ImageJ, or ImageJ Kymograph plugin.
+If you want to measure full frame of the kymograph, simply apply **[Plugins -> Macros -> Measure Velocity from Kymograph: Full Frame]**.
+If you want to measure only part of the kymograph, then select a area by rectangular ROI tool and then **[Plugins -> Macros -> Measure Velocity from Kymograph: ROI]**.
+**[Plugins -> Macros -> Scan Kymograph and Measure - Automatic Multiple Measurements]** will automatically divide the kymograph into segments and sequentially measure the kymograph along the time line.
+[{{ http://cmci.embl.de/dls/Kymoquant/overview_kymoquant.jpg }}]