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Timeline of @cmci_

cmci_ avatar

RT @kjaMartens: We created a very simple method to get high precision and density in spectrally-resolved single-molecule localization micro…
About 1 day, 13 hours ago by: Kota Miura (@cmci_)

cmci_ avatar

RT @random_walker: There’s a reproducibility crisis brewing in almost every scientific field that has adopted machine learning. On July 28,…
About 2 days, 2 hours ago by: Kota Miura (@cmci_)

cmci_ avatar

@AthaleLab @IISERPune @serbonline @DBTIndia @IFCPAR @OfficialSICI @boschindia Congrats Prof. Chait!
About 5 days, 14 hours ago by: Kota Miura (@cmci_)

cmci_ avatar

RT @albertcardona: Video of my #I2K2022 entry-level scripting tutorial for #ImgLib2 using the @FijiSc Script Editor: https://t.co/eastROJm0
About 1 week ago by: Kota Miura (@cmci_)

cmci_ avatar

RT @blg_journal: Biological Imaging has two current calls for papers, please share with your networks: Spatial Analysis in Biological Imag…
About 1 week, 1 day ago by: Kota Miura (@cmci_)

cmci_ avatar

This reference is also interesting. What Is the Region Occupied by a Set of Points? https://t.co/oDajYd4YCt
About 1 week, 1 day ago by: Kota Miura (@cmci_)
downloads:kymoquant
Measure Movement directly from kymograph

Kymoquant

Quantitative analysis of ambiguous patterns in kymograph

Author

Kota Miura
Centre for Molecular and Cellular Imaging (CMCI)
EMBL Heidelberg
miura at embl dot de
Tel: +49 6221 387 404

History

  • 10-03-03 ? first version released

Source

Released under the GNU General Public License.

Requires

ImageJ ver 1.43o or higher (ImageJ, upgrade page).

Installation

Download K_kymoquant.ijm to a place where you can access easily. Install the macro by [Plugins→Macros→Install…].

Description

Measures orientation of kymograph by estimating the direction of image texture. Outputs movement velocity in pixels / frame.

(more explanation on measurement principle is planned here)

Tip: When kymograph traces are steep, analysis results become unstable since calculation of direction involves tanθ. Be sure to capture original sequence with high time resolution if possible.

Work Flow

Kymograph should be prepared by your self either by manually using stack reslicing function in ImageJ, or ImageJ Kymograph plugin.

If you want to measure full frame of the kymograph, simply apply [Plugins → Macros → Measure Velocity from Kymograph: Full Frame].

If you want to measure only part of the kymograph, then select a area by rectangular ROI tool and then [Plugins → Macros → Measure Velocity from Kymograph: ROI].

[Plugins → Macros → Scan Kymograph and Measure - Automatic Multiple Measurements] will automatically divide the kymograph into segments and sequentially measure the kymograph along the time line.

overview_kymoquant.jpg
downloads/kymoquant.txt · Last modified: 2016/05/24 12:46 (external edit)