Using LOCI Bio-Format Command line tools
For converting .lif file to separate tif files:
if LIF file consists of stack series, and to separate them as each tiff stack:
bfconvert laminedapi23012.lif testS%s.tif
then the output would be
testS0.tif testS1.tif testS2.tif ...
… such numbered stacks in the same directory.
All stacks will be extracted as tif stacks with the above command.
To extract only one of the stacks out of .lif file, then specify the series number:
bfconvert -series s laminedapi23012.lif testS%s.tif
If your LIF file consists of stack series, each stack has multiple channels and you want to separate them as each tiff stack with separated channel:
bfconvert laminedapi23012.lif testS%s_C%c.tif
then the output would then be
testS0_C0.tif testS0_C1.tif testS0_C2.tif testS1_C0.tif ...