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RT @kjaMartens: We created a very simple method to get high precision and density in spectrally-resolved single-molecule localization micro…
About 1 day, 14 hours ago by: Kota Miura (@cmci_)

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RT @random_walker: There’s a reproducibility crisis brewing in almost every scientific field that has adopted machine learning. On July 28,…
About 2 days, 3 hours ago by: Kota Miura (@cmci_)

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@AthaleLab @IISERPune @serbonline @DBTIndia @IFCPAR @OfficialSICI @boschindia Congrats Prof. Chait!
About 5 days, 16 hours ago by: Kota Miura (@cmci_)

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RT @albertcardona: Video of my #I2K2022 entry-level scripting tutorial for #ImgLib2 using the @FijiSc Script Editor: https://t.co/eastROJm0
About 1 week ago by: Kota Miura (@cmci_)

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RT @blg_journal: Biological Imaging has two current calls for papers, please share with your networks: Spatial Analysis in Biological Imag…
About 1 week, 1 day ago by: Kota Miura (@cmci_)

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This reference is also interesting. What Is the Region Occupied by a Set of Points? https://t.co/oDajYd4YCt
About 1 week, 1 day ago by: Kota Miura (@cmci_)
documents:frapmanu

FRAP analysis manual

The document is temporally shown via google drive (which is difficult to view). You could adjust the zooming by yourself, or feel free to download via File menu within the interface.

FAQ

My FRAP curve best bits a double exponential fit, but I get two tau values by using a double exponential fit, one small and one large. My question is which tau value do I use? Only the small tau value make sense in my experiments, am I allowed just simply to ignore the large tau value?

The double exponential fits to almost any FRAP curve (even for diffusion only recovery). This causes problem in interpretation of data: it could be that kinetics beihing is nothing to do with the double exponential.

If you know pretty well that the kinetics you are analyzing is indeed a double exponential reaction and doing the experiment correctly, you could interpret that there are reactions with fast and slow kinetics and could use those values to discuss differences.

If such condition is not met, forget about the model behind the recovery. Then all you could do is to compare the half-max time (1/2 tau). This is rather qualitative, but still is valid and scientific.

documents/frapmanu.txt · Last modified: 2020/11/26 09:11 (external edit)