sample image: quantumdots.tif

design a protocol for segmenting quantum dot and write a macro that counts the number of quantum dots in the image.

(Brownian, Cycloid, Sinusoidal …)

Save your macro in the data server. Please do not forget to include your names in the macro file name.

Answers

• XY movement
  • see https://gist.github.com/miura/24a6d91c7358454cb444
• Random Walk
  • see https://gist.github.com/miura/3e1b8bcd73ea864b46ee
  • The function used in this macro could be tested with the following code
    • https://gist.github.com/miura/5592101

Go to section 4.4.7 and do the exercise 4.4.7-1.

Try install and using the following plugin: https://sites.google.com/site/qingzongtseng/piv

Get a vector field data (you need only two frames for this), save the results as a csv file and then analyze the directioanlity in R.