User Tools

Site Tools


Sidebar

Top
Seminar
Courses
Textbooks
Documents
Downloads (-2016)
Downloads (2016-)
Weblog
RSS aggregates
Discussions
Archives


EMBL BioImage Data Analysis

EuBIAS

NEUBIAS

—- Contact
CMCI Alumni
ALMF
EMBL Heidelberg
EMBL Intranet


Popularity Ranking


Timeline of @cmci_

cmci_ avatar

RT @WimJHH: EMBL Heidelberg workshop "Cryo-EM in Industry & Academia", 6-8 February 2019. Success stories, new technologies, industry chall…
About 5 hours, 58 mins ago by: Kota Miura (@cmci_)

cmci_ avatar

@f_levet @BertrandVernay I made it.
About 6 hours, 49 mins ago by: Kota Miura (@cmci_)

cmci_ avatar

RT @haesleinhuepf: One more session on #ImageJ short cuts: SHIFT+U opens the command tree. I didn't know this one. Thanks @BertrandVernay a…
About 7 hours ago by: Kota Miura (@cmci_)

cmci_ avatar

@ManuelTHERY @martinjones78 @NEUBIAS_COST Slowly Manu
About 16 hours, 42 mins ago by: Kota Miura (@cmci_)

cmci_ avatar

@laure_plantard I can’t track JY himself with Trackmate. Metaphysical contradiction.
About 18 hours, 34 mins ago by: Kota Miura (@cmci_)

cmci_ avatar

“2 chapters of my dissertation was only possible with Trackmate”
About 20 hours, 2 mins ago by: Kota Miura (@cmci_)
documents:101124locibioformats_bftoolstip

Using LOCI Bio-Format Command line tools

This is a command line examples of "bfconvert" tool from LOCI Bioformats1).

For converting .lif file to separate tif files:

if LIF file consists of stack series, and to separate them as each tiff stack:

bfconvert laminedapi23012.lif testS%s.tif

then the output would be

 testS0.tif
 testS1.tif
 testS2.tif
 ...

… such numbered stacks in the same directory.

All stacks will be extracted as tif stacks with the above command.
To extract only one of the stacks out of .lif file, then specify the series number:

bfconvert -series s laminedapi23012.lif testS%s.tif

If your LIF file consists of stack series, each stack has multiple channels and you want to separate them as each tiff stack with separated channel:

bfconvert laminedapi23012.lif testS%s_C%c.tif

then the output would then be

 testS0_C0.tif
 testS0_C1.tif
 testS0_C2.tif
 testS1_C0.tif
 ...
1)
within embl network, this tool is reachable by /g/almf/software/bin2/bfconvert
documents/101124locibioformats_bftoolstip.txt · Last modified: 2016/05/24 05:46 (external edit)