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Timeline of @cmci_

cmci_ avatar

Miyo Terao Morita showing analysis of gravitropism using centrifuge microscope they newly developed. I only know… https://t.co/Atj9E4QJAY
About 9 mins, 36 secs ago by: Kota Miura (@cmci_)

cmci_ avatar

@KeikoUTorii ... which then became the cover page of Development cell. convincing. https://t.co/XBgiqJpHog
About 1 hour, 6 mins ago by: Kota Miura (@cmci_)

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@KeikoUTorii model-based hypothesis lead to experiment results with single guard cell development or multiple >2 gu… https://t.co/oGhh4NJMPh
About 1 hour, 14 mins ago by: Kota Miura (@cmci_)

cmci_ avatar

now, @KeikoUTorii on stomata development - how very specific cell fate decisions are made, with proper spacing bet… https://t.co/XoR8tpB5WR
About 1 hour, 31 mins ago by: Kota Miura (@cmci_)

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@YvonJaillais talk on nanoclustering of ROP6, great analyses using various approaches to assess protein mobility… https://t.co/WHrzBsWTYJ
About 1 hour, 47 mins ago by: Kota Miura (@cmci_)

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RT @haesleinhuepf: I need to add this one, because I believe in the power of beautiful data. Same optics, just earlier stage in development…
About 13 hours, 19 mins ago by: Kota Miura (@cmci_)
downloads:hyperstackexporttoimaris

Export Hyperstack to Imaris

Author

Kota Miura
Centre for Molecular and Cellular Imaging (CMCI)
EMBL Heidelberg
miura at embl dot de
Tel: +49 6221 387 404

History

  • 10-08-06 First Version.

Source

Released under the GNU General Public License.

Requires

ImageJ ver 1.37j or higher (ImageJ, upgrade page).

A commercial software Imaris (Bitplane) ver 7.0.0 and its optional module ImarisXT.

Installation

You need to install several files.

  1. Download ImarisPlugin3_.jar and place the file in the plugin folder of ImageJ.
  2. Download jacob-1.15-M3.zip from JACOB site (JACOB bridge is a module that bridges between Java and Windows application). Unzip the file and then
    • place jacob-1.15-M3-x86.dll (if your machine is 64-bit, then jacob-1.15-M3-x64.dll) in a directory where system path is set in your machine. In my case, I copied it under C:\Sun\SDK\jdk\jre\bin.
    • place jacob.jar under C:\Sun\SDK\jdk\jre\lib\ext, where java path is set.

Description

Exports multidimensional stack opened in ImageJ directly to Imaris, so that 3D time series with multiple channels could be visualized and inspected using useful interface of Imaris. It works with 8-bit and 16-bit stacks. 32-bit stack exporting should work but not tested.

Following types of stacks could be exported:

  • Z stack
  • cz stack (up to 4 channels)
  • RGB - Z stack
    • each color channel will be treated as a single channel)
  • ZT stack
  • CZT stack
  • RGB - ZT stack
    • each color channel will be treated as a single channel)

In case of CZ, ZT, CZT, RGB-ZT stacks, stack should be already be converted to Hyperstack (Image → Stack → Stack to HyperStack). You should also be sure that dimensional sizes in [Image → Image Properties] are correctly set, since plugin reads dimensional information from this.

Work Flow

  • Open a hyperstack in ImageJ.
  • Do [Plugins → Imaris → Hyperstack Exporter…]. “Imaris Interface” window appears.
  • Click “Start Imaris”. Imaris application starts up.
  • In the panel “Stack Export to Imaris”, click appropriate button that matches with the dimension of your stack. Then wait for the exporting to finish.
  • You could close this “Imaris Interface” window. Although there is check button saying “Exit Imaris when closing this dialog”, Imaris survives even when ImageJ is terminated.

Development

Acknowledgement

This plugin was written on top of example java application “HelloImaris.java” written by Volker Baecker, which is available in the Bitplane website.

downloads/hyperstackexporttoimaris.txt · Last modified: 2016/05/24 05:46 (external edit)