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clij moved to a new house https://t.co/XJ0YBhE2jE
About 2 hours, 49 mins ago by: Kota Miura (@cmci_)

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RT @regagino: My postdoc work with Sarah Woolner's lab @FBMH_UoM is out @CellReports! Decoupling the Roles of Cell Shape and Mechanical St…
About 18 hours, 10 mins ago by: Kota Miura (@cmci_)

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RT @sbarolo: #WomeninSTEM get a lot of “Reply Guys” who repeat the same unhelpful comments. @shrewshrew and I (a woman & a man in science)…
About 18 hours, 11 mins ago by: Kota Miura (@cmci_)

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RT @cohenlaboratory: Female with microscope? Add yourself to the list! https://t.co/gN3ThsklUj
About 1 day, 17 hours ago by: Kota Miura (@cmci_)

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RT @Dr_Fluo: The mesmerizing real-time shuttling of protoplasm within the plasmodial syncitium of the #Physarum #SlimeMold. Scarily dynamic…
About 1 day, 21 hours ago by: Kota Miura (@cmci_)

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I did the same on Sunday hearing the news. Many of the monologues of the people were a bit remote to me when I was… https://t.co/5dZWBQPMv5
About 1 day, 21 hours ago by: Kota Miura (@cmci_)
downloads:kymoquant
Measure Movement directly from kymograph

Kymoquant

Quantitative analysis of ambiguous patterns in kymograph

Author

Kota Miura
Centre for Molecular and Cellular Imaging (CMCI)
EMBL Heidelberg
miura at embl dot de
Tel: +49 6221 387 404

History

  • 10-03-03 ? first version released

Source

Released under the GNU General Public License.

Requires

ImageJ ver 1.43o or higher (ImageJ, upgrade page).

Installation

Download K_kymoquant.ijm to a place where you can access easily. Install the macro by [Plugins→Macros→Install…].

Description

Measures orientation of kymograph by estimating the direction of image texture. Outputs movement velocity in pixels / frame.

(more explanation on measurement principle is planned here)

Tip: When kymograph traces are steep, analysis results become unstable since calculation of direction involves tanθ. Be sure to capture original sequence with high time resolution if possible.

Work Flow

Kymograph should be prepared by your self either by manually using stack reslicing function in ImageJ, or ImageJ Kymograph plugin.

If you want to measure full frame of the kymograph, simply apply [Plugins → Macros → Measure Velocity from Kymograph: Full Frame].

If you want to measure only part of the kymograph, then select a area by rectangular ROI tool and then [Plugins → Macros → Measure Velocity from Kymograph: ROI].

[Plugins → Macros → Scan Kymograph and Measure - Automatic Multiple Measurements] will automatically divide the kymograph into segments and sequentially measure the kymograph along the time line.

overview_kymoquant.jpg
downloads/kymoquant.txt · Last modified: 2016/05/24 05:46 (external edit)