Color coding traces by time.

Kota Miura
Centre for Molecular and Cellualr Imaging (CMCI)
EMBL Heidelberg
miura at embl dot de
Tel: +49 6221 387 404

• 08-02-12 created ver.1 K_TimeRGBcolorcode.ijm
• 08-02-13 stack slice range option added.
  • time color code scale option added.
  • future probable addiition: none-linear assigning of gray intensity to color intensity
  • → but this is same as doing contrast enhancement before processing.
• 10-12-06 … is now part of Fiji. See details in the Fiji web site.

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Released under the GNU General Public License.

GitHub

ImageJ ver 1.34j or higher (ImageJ, upgrade page).

It comes with ImageJ / Fiji, so you do not need to install it.

Download K_TimeRGBcolorcode.ijm to a place where you can access easily. Install the macro by [Plugins→Macros→Install…].

Temporal changes in position of object will be color coded.
For example, sequece such as

will be converted to color coded stack

Max intensity projection of this sequence yields

Coding table will also be created (check the option).

1. Open a stack (8 bit or 16 bit)
2. Run the macro
3. In the dialog choose one of the LUT for time coding.?Select frame range (default is full). Check if you want to have color scale bar.