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EMBL BioImage Data Analysis

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Yoshikatsu Sato, the head of microscope facility @NagoyaITbM presenting practicals #EMBOLivePlant https://t.co/SVrkfOKQCn
About 37 mins, 3 secs ago by: Kota Miura (@cmci_)

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Miyo Terao Morita showing analysis of gravitropism using centrifuge microscope they newly developed. I only know… https://t.co/Atj9E4QJAY
About 1 hour, 9 mins ago by: Kota Miura (@cmci_)

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@KeikoUTorii ... which then became the cover page of Development cell. convincing. https://t.co/XBgiqJpHog
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@KeikoUTorii model-based hypothesis lead to experiment results with single guard cell development or multiple >2 gu… https://t.co/oGhh4NJMPh
About 2 hours, 13 mins ago by: Kota Miura (@cmci_)

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now, @KeikoUTorii on stomata development - how very specific cell fate decisions are made, with proper spacing bet… https://t.co/XoR8tpB5WR
About 2 hours, 30 mins ago by: Kota Miura (@cmci_)

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@YvonJaillais talk on nanoclustering of ROP6, great analyses using various approaches to assess protein mobility… https://t.co/WHrzBsWTYJ
About 2 hours, 46 mins ago by: Kota Miura (@cmci_)
shared:bias2013:assignments

Assignments

module 1 and 2

1. Quantum dot counting (planned, but omitted)

sample image: quantumdots.tif

design a protocol for segmenting quantum dot and write a macro that counts the number of quantum dots in the image.

2. Modify dot movement macro as you like.

(Brownian, Cycloid, Sinusoidal …)

Save your macro in the data server. Please do not forget to include your names in the macro file name.

Answers

module 4

Relative Movement

Go to section 4.4.7 and do the exercise 4.4.7-1.

PIV based anaysis (omitted)

Try install and using the following plugin: https://sites.google.com/site/qingzongtseng/piv

Get a vector field data (you need only two frames for this), save the results as a csv file and then analyze the directioanlity in R.

shared/bias2013/assignments.txt · Last modified: 2017/06/06 20:12 by kota