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RT @BoSoxBioBeth: 2 days left to submit an abstract proposal! We want all your best ideas on image analysis!
About 8 hours, 18 mins ago by: Kota Miura (@cmci_)

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RT @ManuelTHERY: Here we are. The combination of biochemically controled differentiation of iPS cells with microprinting to direct cell sel…
About 8 hours, 18 mins ago by: Kota Miura (@cmci_)

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RT @haesleinhuepf: "Do you also spend a substantial amount of your time waiting for automated image analysis to finish? [...] And, then, th…
About 19 hours, 44 mins ago by: Kota Miura (@cmci_)

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RT @ALM_i3S: Do you want to extract quantitative data from images and automate this process by learning ImageJ Macro Language? Apply to t…
About 1 day, 14 hours ago by: Kota Miura (@cmci_)

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RT @martinjones78: Very interesting #BioImageAnalysis PhD position! https://t.co/85QLBKiANx
About 1 day, 14 hours ago by: Kota Miura (@cmci_)

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RT @jobRxiv: #ScienceJobs: Image Analyst / Python programmer Francis Crick Institute (@TheCrick), London, UK https://t.co/T7cDhv9PX0 #P…
About 1 day, 14 hours ago by: Kota Miura (@cmci_)
blogtng:2010-05-06:bleach_correction_2

Bleach Correction (2)

As I test through different method further

  1. Phair's method (or simple ratio)
  2. Exponential decay fitting (or exponential ratio, I call)
  3. Histogram streching,

all these methods are not really satisfactory. so I kept on searching for other methods and I tested something not used often, which is called “Histgram Matching”, and it seems to work better. Bleached images are better be blurred a bit. I might try implementing it as another method for bleaching correction.

Followings are links related to this method, which I referred to.

Histogram equalizaiton theory (by R. Fisher, S. Perkins, A. Walker and E. Wolfart.@ Image Processing Learning Resources).

I also refrred to Digital Image Processing using Matlab“ but it was too simply formulated (and, the matlab function which should be used is indicated but too short).

Paper: “A statistical approach for intensity loss compensation of confocal microscopy images”, Gopinath et al (2007) J. of microscopy ( Link). This paper tries to correct the acquistion bleaching while the image itself is changing a lot (the sample seems to be internalizing cell surface recepter, so from diffuse signal to dotty signals). In case of sequences with less changes in signal shape, the problem is more simple and straightforward, but such dynamic version should be already in our sight. (by the way, I am always amazed by works which Luby-Phelps is involved in. When I found the name on this paper, amazed again…).

Point Operations
CMPUT 206, Instructor: NilanjanRay
(powerpoint slides in PDF)

Java library: source for historam matching is in Burger & Burge website, chapter 5.
"Digital Image Processing: An algorithmic introduction uing Java"

blogtng/2010-05-06/bleach_correction_2.txt · Last modified: 2016/05/24 05:46 (external edit)