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EMBL BioImage Data Analysis

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Timeline of @cmci_

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RT @biomicugm: Topics: Biostatistics, computational evolutionary biology, computational genomics and proteomics, graph models, image analys…
About 4 days, 16 hours ago by: Kota Miura (@cmci_)

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RT @JustinMCrocker: We are looking for an interdisciplinary EIPOD postdoc to join our group @EMBL! Potential projects with @Eileen_Furlong,…
About 4 days, 16 hours ago by: Kota Miura (@cmci_)

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RT @BioImagingUK: This maybe of interest to the @NEUBIAS_COST @openmicroscopy communities #imageAnalysis #imagingScientist https://t.co/I5
About 4 days, 16 hours ago by: Kota Miura (@cmci_)

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RT @BioImagingUK: Software developer position on large image data analysis - working closely with the lab of @bayraktar_lab @sangerinstitut…
About 1 week, 2 days ago by: Kota Miura (@cmci_)

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RT @AssafZaritsky: Modeling the interplay of lamellipodial motion and the intercellular actomyosin cable during epithelial wound healing h…
About 1 week, 2 days ago by: Kota Miura (@cmci_)

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RT @jytinevez: @florianjug says be careful when using #CARE for intensity based quantification; CARE restoration is like a non linear trans…
About 1 week, 4 days ago by: Kota Miura (@cmci_)
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Algorithm FRAP Fiji ImageJ ImageJ Plugin ImageJ Plugin 3Dviewer Imaris Java Javascript Python R bias blog dokuwiki fiji google imagej java libraries matlab meetings neubias news papers python references software webadmin




CMCI weblog

Matlab Web Tutorials

I was asked about available web tutorials to learn Matlab from scratch: here is some links


Start

get to know Matlab

choose several from Matlab tutorials
http://www.mathworks.com/academia/student_center/tutorials/launchpad.html

or if you know a bit about command line interface

http://www.mit.edu/people/abbe/matlab/lec1.html http://www.mit.edu/people/abbe/matlab/lec2.html

should be quicker.

You could also go through a list of Matlab tutorials
http://www.duke.edu/~hpgavin/matlab.html

Image Processing

Matlab - R

very well documentation comparing Matlab and R.

http://www.math.umaine.edu/~hiebeler/comp/matlabR.html

Linked PDF contains huge number of functions in R equivalent to Matlab functions. Some useful discussions on difference between R and matlab could be found in

http://stackoverflow.com/questions/1738087/what-can-matlab-do-that-r-cannot-do

Writing Matlab code for ImarisXT

Getting Imaris object is pretty similar (see previous notes).

Found several links besides Bitplane site.

Aaron Christian Pontis Qu http://www.scs2.net/home/index.php?option=com_content&view=article&id=46:qu-for-matlab&catid=34:qu&Itemid=55&showall=1

Pretty much of communication with Imaris.

  • Sending 4D stack (successful with 8 z-slices, 46 timepoints tiff hyperstack)
  • Retrieving spots detected in Imaris back to matlab (I waited 5 minutes but currently not responding… finally, I got all the spots, none-filtered. It took time probably because I did not filter).

How to write Matlab Code for ImarisXT: a Short Tutorial http://sybil.ece.ucsb.edu/pages/imarisxt.html

this is a bit outdated.


I will further try doing something like

  • set parameters for spot detection from Matlab or Java
  • do spot analysis in Imaris using above setting
  • fetch the results of detected spots from Imaris to Matlab or Java
  • evaluate the quality. Change spot detecton parameter
  • do spot analysis again with updated parameter.
  • this looping goes on until certain satisfactory value is achieved, walk through parameter space.

For this, image stack itself stays in Imaris. Only parameter setting is exported from java/matlab to imaris, and analysis results are thrown back to matlab/java.

entry generator

Weblog Archive

blogtng/blogtop.txt · Last modified: 2016/05/24 05:46 (external edit)